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Tissue/ cell preparation: Mouse leukocyte cell line 32D.
This antibody inhibits the aggregation of L cells transfected with a variant form of CD31.
Our Abpromise guarantee covers the use of ab24960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration.|
Flow cytometry image of ab24960 staining mouse embryonic stem cells after trypsinization from tissue culture plastic. Live cells were gated using forward and side scatter. The primary antibody was diluted 1200 and incubated with the sample for 1 hr at 4°C. A PE-Cy5® Streptavidin fusion protein at a 1/250 dilution, was used instead of a secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"