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Mouse leukocyte cell line 32D
ab25644 inhibits the aggregation of L cells transfected with a variant form of CD31.
Our Abpromise guarantee covers the use of ab25644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells. ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
ab25644 staining CD31 in Mouse stem cell derived endothelial cells by Flow Cytometry. Cells were detached, washed, centrifuged and resuspended in 10% FCS/PBS/0.1 mM EDTA and incubated for 30 minutes on ice. The sample was incubated with the primary antibody (0.5 µg per 1 x 106 cells) for 30 minutes at 4°C in the dark. An Isotype-matched IgG-PE antibody was used as control. After adding 1.5ml cold 5% FCS/PBS/0.1 mM EDTA each tube was mixed by inversion and centrifuged; the supernatant was aspirated. After repeat washing the cells were resuspended in 400µl cold 1% FCS/PBS/0.1 mM EDTA and analyzed.