Overview

  • Product nameAnti-CD31 antibody
    See all CD31 primary antibodies
  • Description
    Rabbit polyclonal to CD31
  • SpecificityDetects endogenous levels of total CD31 protein.
  • Tested applicationsSuitable for: ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from Human CD31 around the phosphorylation site of tyrosine 713 (T-V-YP-S-E).

  • Positive control
    • Human brain tissue. Thyroid, lung, placenta tissue.

Applications

Our Abpromise guarantee covers the use of ab59251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-P
IHC-Fr
  • Application notesELISA: 1/20000.
    IHC-Fr: Use at a concentration of 5 - 10 µg/ml (PMID 19888452).
    IHC-P: 1/50 - 1/100.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInduces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
    • Tissue specificityExpressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
    • Sequence similaritiesContains 6 Ig-like C2-type (immunoglobulin-like) domains.
    • DomainThe Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
    • Post-translational
      modifications
      Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
    • Cellular localizationMembrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adhesion molecule antibody
      • CD31 antibody
      • CD31 antigen antibody
      • CD31 EndoCAM antibody
      • EndoCAM antibody
      • FLJ34100 antibody
      • FLJ58394 antibody
      • GPIIA antibody
      • GPIIA' antibody
      • PECA1 antibody
      • PECA1_HUMAN antibody
      • Pecam 1 antibody
      • PECAM 1 CD31 EndoCAM antibody
      • PECAM antibody
      • PECAM-1 antibody
      • Pecam1 antibody
      • Platelet and endothelial cell adhesion molecule 1 antibody
      • Platelet endothelial cell adhesion molecule antibody
      • Platelet/endothelial cell adhesion molecule 1 antibody
      see all

    Anti-CD31 antibody images

    • ab59251 at 1/50 dilution staining CD31 in Human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide

    References for Anti-CD31 antibody (ab59251)

    This product has been referenced in:
    • Roland CL  et al. Cytokine levels correlate with immune cell infiltration after anti-VEGF therapy in preclinical mouse models of breast cancer. PLoS One 4:e7669 (2009). IHC-Fr ; Mouse . Read more (PubMed: 19888452) »
    • Serikov V  et al. Human term placenta as a source of hematopoietic cells. Exp Biol Med (Maywood) 234:813-23 (2009). Flow Cyt ; Human . Read more (PubMed: 19429852) »

    See all 2 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (A431 Cells)
    Loading amount 5 µg
    Specification A431 Cells
    Gel Running Conditions Reduced Denaturing (4-12%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 15 2010

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Liver - Carcinoma)
    Specification Liver - Carcinoma
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid pH=6,0
    Permeabilization No
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 14 2010

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (kidney)
    Specification kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 26 2008

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (kidney)
    Specification kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 26 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"