Overview

  • Product nameAnti-CD31 antibody [P2B1]
    See all CD31 primary antibodies
  • Description
    Mouse monoclonal [P2B1] to CD31
  • Tested applicationsSuitable for: ICC/IF, IHC - Wholemount, WB, IPmore details
    Unsuitable for: IHC-Fr or IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
    Does not react with: Cow
  • Immunogen

    Tissue, cells or virus corresponding to Human CD31.

  • General notes

    ab24590 activates the binding of human T-lymphocytes to endothelial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab24590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC - Wholemount 1/100.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 83 kDa).

We recommend using 3% milk as the blocking agent for Western blot.

IP 1/1000.
  • Application notesIs unsuitable for IHC-Fr or IHC-P.
  • Target

    • FunctionInduces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
    • Tissue specificityExpressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
    • Sequence similaritiesContains 6 Ig-like C2-type (immunoglobulin-like) domains.
    • DomainThe Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
    • Post-translational
      modifications
      Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
    • Cellular localizationMembrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adhesion molecule antibody
      • CD31 antibody
      • CD31 antigen antibody
      • CD31 EndoCAM antibody
      • EndoCAM antibody
      • FLJ34100 antibody
      • FLJ58394 antibody
      • GPIIA antibody
      • GPIIA' antibody
      • PECA1 antibody
      • PECA1_HUMAN antibody
      • Pecam 1 antibody
      • PECAM 1 CD31 EndoCAM antibody
      • PECAM antibody
      • PECAM-1 antibody
      • Pecam1 antibody
      • Platelet and endothelial cell adhesion molecule 1 antibody
      • Platelet endothelial cell adhesion molecule antibody
      • Platelet/endothelial cell adhesion molecule 1 antibody
      see all

    Anti-CD31 antibody [P2B1] images

    • Positive immunocytochemical staining of human primary choroidal endothelial cells using ab24590 at a dilution of 1/100. The tissue was fixed with paraformaldehyde and the antibody was incubated for 3 hours. An Alexa Fluor® 488 goat anti-mouse antibody was used as a secondary antibody.

      See Abreview

    • Images of isolated capillaries triple labeled for EC marker protein CD31 (red channel), PC marker protein NG-2 (green channel), and Hoechst for nuclei (blue channel).

      Cochlear stria vascularis capillaries were isolated from 6-week-old CBA/CaJ mice. The capillary isolation was achieved with a special procedure we developed and named the “sandwich-dissociation” method. The tissue samples were fixed in 4% paraformaldehyde at 4°C for 2 h, washed in PBS for 30 min, permeabilized in 0.5% Triton X-100, then immunoblocked in a solution of 10% goat serum in 1% bovine albumin in PBS for 30 min. The specimens were incubated with primary antibodies and secondary antibodies (goat Alexa Fluo 488-conjugated anti-mouse and anti-rabbit IgG antibodies).

    • ab24590 staining CD31 in the HEK 293T cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton and blocked with 3% BSA for 1 hour at RT. Samples were incubated with primary antibody (1/50 in PBS + 3% BSA) for 1 hour. A Alexa Fluor® 555-conjugated Donkey anti-mouse polyclonal was used as the secondary antibody (1/500).

      See Abreview

    • All lanes : Anti-CD31 antibody [P2B1] (ab24590) at 5 µg/ml

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) WCL
      Lane 2 : THP1 (Human acute monocytic leukemia cell line) WCL
      Lane 3 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
      Lane 4 : Human Platelets

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size : 83 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)


      Exposure time : 4 minutes

      We recommend using 3% milk as the blocking agent for Western blot.

    References for Anti-CD31 antibody [P2B1] (ab24590)

    This product has been referenced in:
    • Lee J  et al. Tissue Transglutaminase Activates Cancer-Associated Fibroblasts and Contributes to Gemcitabine Resistance in Pancreatic Cancer. Neoplasia 18:689-698 (2016). Read more (PubMed: 27792935) »
    • Li J  et al. Fibrin patch-based insulin-like growth factor-1 gene-modified stem cell transplantation repairs ischemic myocardium. Exp Biol Med (Maywood) 240:585-92 (2015). IHC-P . Read more (PubMed: 25767192) »

    See all 26 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Pig Cell lysate - whole cell (PAE cells)
    Specification PAE cells
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

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    Submitted Jan 21 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Fish Tissue sections (Heart)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate pH6.0
    Permeabilization Yes - Triton X
    Specification Heart
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 24 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Human fibromatosis)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
    Permeabilization Yes - Tween-20
    Specification Human fibromatosis
    Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

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    Submitted Dec 15 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (mouse liver)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Permeabilization Yes - Tween-20
    Specification mouse liver
    Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative Formaldehyde
    Username

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    Submitted Dec 08 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample Human Cell (HEK 293T)
    Specification HEK 293T
    Permeabilization Yes - Triton 1%
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Nov 18 2014

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Peripheral blood lymphocyte (PBL))
    Specification Peripheral blood lymphocyte (PBL)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative Acetone
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    Abcam user community

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    Submitted Oct 22 2014

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing
    Sample Rat Tissue lysate - whole (liver)
    Specification liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    Submitted Oct 22 2014

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing
    Sample Mouse Tissue lysate - whole (liver)
    Specification liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

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    Submitted Oct 22 2014

    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Liver)
    Specification Liver
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative Acetone
    Username

    Abcam user community

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    Submitted Oct 20 2014

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample Mouse Tissue sections (Liver)
    Specification Liver
    Permeabilization No
    Fixative Acetone
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    Submitted Oct 20 2014

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (4-12%)
    Sample Human Tissue lysate - whole (liver)
    Specification liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

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    Submitted Oct 14 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Human tumor xenographted into a mouse)
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Trypsin
    Permeabilization No
    Specification Human tumor xenographted into a mouse
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
    Username

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    Submitted Jul 15 2014

    Application IHC - Wholemount
    Sample Rat Tissue (retina whole mount)
    Specification retina whole mount
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    Submitted May 02 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (omentum)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate pH=6
    Permeabilization No
    Specification omentum
    Blocking step normal goat serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Fixative Paraformaldehyde
    Username

    Miss. Berna Senturk

    Verified customer

    Submitted Nov 01 2013

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application IHC - Wholemount
    Sample Human Tissue (vessel)
    Specification vessel
    Username

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    Submitted May 27 2013



    It is an interesting situation and I'm glad you mentioned it. The theoretical mass of CD31 is about 86kDa. However CD31 can be extensively modified post-translationally. These modifications include phosphorylation, palmitoylation and glycosyl...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Human dermal mivrovascular endothelial cell)
    Loading amount 30 µg
    Specification Human dermal mivrovascular endothelial cell
    Treatment 10ng TNF-alpha for 48hrs
    Gel Running Conditions Reduced Denaturing (9% SDS-PAGE)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Mar 18 2013

    There are some isoforms of CD31 in human and mouse.


    Human:http://www.uniprot.org/uniprot/P16284
    Mouse:http://www.uniprot.org/uniprot/Q08481



    Based on your results, I would like to make some proposal.
    -It may be t...

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    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Liver)
    Specification Liver
    Fixative Acetone
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

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    Submitted Dec 26 2012

    I have checked the alignment of the immunogen forab24590 with the chickenPCAM1 sequence. I am sorry this is low at 31%. Therefore, I am sorry to confirm that we would suggest the antibody would not be likely to detect in Chicken, although this has not ...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Brain)
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Trypsin
    Permeabilization Yes - Triton X
    Specification Brain
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Kate Schunke

    Verified customer

    Submitted Aug 24 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Cryosection-Heart)
    Specification Cryosection-Heart
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.05% Triton
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

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    Submitted Jul 27 2012

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Kidney)
    Specification Kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate pH6.0
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

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    Submitted Feb 15 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HepG2)
    Loading amount 50 µg
    Specification HepG2
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    Submitted Dec 01 2011

    I can confirm that CD31 is known to be a good endothelial marker. http://en.wikipedia.org/wiki/CD31 The following publications also indicate the staining of CD31 as a good marker for endothelial vascularisation. However, as I am not an expert in th...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (Liver)
    Loading amount 50 µg
    Specification Liver
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 28 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HepG2)
    Loading amount 50 µg
    Specification HepG2
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

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    Submitted Oct 11 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Liver)
    Loading amount 50 µg
    Specification Liver
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

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    Submitted Aug 26 2011

    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Liver)
    Specification Liver
    Fixative Acetone
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Dr. Shakil Ahmad

    Verified customer

    Submitted Aug 11 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (Liver)
    Loading amount 50 µg
    Specification Liver
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Shakil Ahmad

    Verified customer

    Submitted Aug 05 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HUVEC)
    Specification HUVEC
    Fixative Formaldehyde
    Permeabilization Yes - 0.1% triton-X
    Blocking step Goat Serum (Invitrogen, prediluted) as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Karyn O'Connell

    Verified customer

    Submitted Oct 13 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (kidney)
    Specification kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 21 2008

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (adult kidney)
    Specification adult kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 26 2008

    Application Immunocytochemistry
    Sample Mouse Cell (choriocapillaris ECs)
    Specification choriocapillaris ECs
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5%
    Username

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    Submitted Feb 08 2006

    Application Immunocytochemistry
    Sample Human Cell (choroidal endothelium)
    Specification choroidal endothelium
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3%
    Username

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    Submitted Jan 25 2006

    Application Immunocytochemistry
    Sample Rat Cell (dermal endothelium)
    Specification dermal endothelium
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4%
    Username

    Abcam user community

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    Submitted Dec 07 2005

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Cow Cell (retinal endothelium)
    Specification retinal endothelium
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3%
    Username

    Abcam user community

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    Submitted Nov 21 2005

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
    Sample Mouse Tissue lysate - whole (Brain)
    Specification Brain
    Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

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    Submitted May 28 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"