Overview

  • Product nameAnti-CD31 antibody [TLD-3A12]
    See all CD31 primary antibodies
  • Description
    Mouse monoclonal [TLD-3A12] to CD31
  • Specificityab64543 recognises rat PECAM-1 (CD31). It also partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen.
  • Tested applicationsSuitable for: WB, IHC-Fr, Flow Cyt, ICC/IF, ELISAmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Rat
    Does not react with: Human
  • Immunogen

    Activated, Lewis rat derived microglial cells

  • Positive control
    • Rat spleen; activated microglial cells.
  • General notes


    This antibody has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen.

Properties

Applications

Our Abpromise guarantee covers the use of ab64543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.
IHC-Fr 1/10 - 1/100. A fixation with acetone or methanol is recommended. Do not fix with PFA.
Flow Cyt Use 10µl for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 20813915
ELISA Use at an assay dependent concentration.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionInduces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
    • Tissue specificityExpressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
    • Sequence similaritiesContains 6 Ig-like C2-type (immunoglobulin-like) domains.
    • DomainThe Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
    • Post-translational
      modifications
      Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
    • Cellular localizationMembrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adhesion molecule antibody
      • CD31 antibody
      • CD31 antigen antibody
      • CD31 EndoCAM antibody
      • EndoCAM antibody
      • FLJ34100 antibody
      • FLJ58394 antibody
      • GPIIA antibody
      • GPIIA' antibody
      • PECA1 antibody
      • PECA1_HUMAN antibody
      • Pecam 1 antibody
      • PECAM 1 CD31 EndoCAM antibody
      • PECAM antibody
      • PECAM-1 antibody
      • Pecam1 antibody
      • Platelet and endothelial cell adhesion molecule 1 antibody
      • Platelet endothelial cell adhesion molecule antibody
      • Platelet/endothelial cell adhesion molecule 1 antibody
      see all

    Anti-CD31 antibody [TLD-3A12] images

    • Immunohistochemical staining of rat brain cryosection with Mouse anti Rat CD31 (ab64543)
    • ab64543 staining CD31 in rat brain tissue by Immunohistochemistry (Frozen sections). The sections were fixed in paraformaldehyde prior to blocking with 5% donkey serum, BSA 1%, Triton 1.5% for 2 hours at room temperature. The primary antibody was diluted 1/200 in PBS/5% Donkey serum and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 555-conjugated donkey anti-mouse polyclonal was used as the secondary antibody, diluted 1/200.

      See Abreview

    • Immunohistochemical staining of rat spleen cryosection with Mouse anti Rat CD31 (ab64543)

    References for Anti-CD31 antibody [TLD-3A12] (ab64543)

    This product has been referenced in:
    • Zhang J  et al. Dimethyloxaloylglycine Promotes the Angiogenic Activity of Mesenchymal Stem Cells Derived from iPSCs via Activation of the PI3K/Akt Pathway for Bone Regeneration. Int J Biol Sci 12:639-52 (2016). IHC-P . Read more (PubMed: 27194942) »
    • Huang SP  et al. Promotion of wound healing using adipose-derived stem cells in radiation ulcer of a rat model. J Biomed Sci 20:51 (2013). Read more (PubMed: 23876213) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (liver)
    Gel Running Conditions Reduced Denaturing
    Loading amount 25 µg
    Specification liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 08 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Heart, skin, bone, cartilage)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH 6.0, also Tris/EDTA pH 9.0
    Permeabilization No
    Specification Heart, skin, bone, cartilage
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative Formaldehyde
    Username

    Mr. Chris Erickson

    Verified customer

    Submitted Apr 14 2016

    Merci pour votre appel la semaine dernière et toutes mes excuses pour le retard de ma réponse.

    J'ai trouvé un anticorps dans notre catalogue qui correspond à vos besoins. Effectivement, le ab21287 est un anticorps ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Sample Rat Tissue sections (brain)
    Specification brain
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Ms. Valley Lasry

    Verified customer

    Submitted Aug 04 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"