• Product nameAnti-CD31 antibody [TLD-3A12] (FITC)
    See all CD31 primary antibodies
  • Description
    Mouse monoclonal [TLD-3A12] to CD31 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityAb33858 recognises CD31 expressed primarily on endothelial cells, platelets and leucocytes.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
    Does not react with: Human
  • Immunogen

    Tissue/ cell preparation: Activated, Lewis rat derived microglial cells.

  • General notes

    This clone (TLD 3A12) has been shown to partially block the proliferative response of antigen specific CD4+ T cells to antigen presenting cells and relevant antigen.



Our Abpromise guarantee covers the use of ab33858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Use 10µl for labeling 106 cells in 100µl.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionInduces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • Tissue specificityExpressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • Sequence similaritiesContains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainThe Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • Post-translational
    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • Cellular localizationMembrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adhesion molecule antibody
    • CD31 antibody
    • CD31 antigen antibody
    • CD31 EndoCAM antibody
    • EndoCAM antibody
    • FLJ34100 antibody
    • FLJ58394 antibody
    • GPIIA antibody
    • GPIIA' antibody
    • PECA1 antibody
    • PECA1_HUMAN antibody
    • Pecam 1 antibody
    • PECAM 1 CD31 EndoCAM antibody
    • PECAM antibody
    • PECAM-1 antibody
    • Pecam1 antibody
    • Platelet and endothelial cell adhesion molecule 1 antibody
    • Platelet endothelial cell adhesion molecule antibody
    • Platelet/endothelial cell adhesion molecule 1 antibody
    see all

References for Anti-CD31 antibody [TLD-3A12] (FITC) (ab33858)

This product has been referenced in:
  • Li YL  et al. Enhanced Protective Effect of the Combination of Uncaria and Semen Raphani on Vascular Endothelium in Spontaneously Hypertensive Rats. Evid Based Complement Alternat Med 2015:358352 (2015). Read more (PubMed: 26539222) »
  • Meier DT  et al. Matrix Metalloproteinase-9 Protects Islets from Amyloid-induced Toxicity. J Biol Chem 290:30475-85 (2015). Read more (PubMed: 26483547) »

See all 5 Publications for this product

Product Wall

This antibody does not require any permeabilization for detection, so although we have not identified the epitope of this antibody, we can assume that it is extracellular.

The light chain has not been determined for this antibody. The isotype control that you have just purchased should still be fine and is the best control that can be selected in this case.