Overview

  • Product nameAnti-CD31 antibody [WM59]
    See all CD31 primary antibodies
  • Description
    Mouse monoclonal [WM59] to CD31
  • SpecificityThis antibody recognises the human CD31 cell surface antigen. CD31 is also known as PECAM-1 and has a MW of 130 kD. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus Monkey, Rhesus monkey
  • EpitopeMaps to domain 2 of CD31 as defined on p364 of Leukocyte Typing VI.

Properties

Applications

Our Abpromise guarantee covers the use of ab218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/100.
IP Use at an assay dependent concentration.

Target

  • FunctionInduces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • Tissue specificityExpressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • Sequence similaritiesContains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainThe Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • Post-translational
    modifications
    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • Cellular localizationMembrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adhesion molecule antibody
    • CD31 antibody
    • CD31 antigen antibody
    • CD31 EndoCAM antibody
    • EndoCAM antibody
    • FLJ34100 antibody
    • FLJ58394 antibody
    • GPIIA antibody
    • GPIIA' antibody
    • PECA1 antibody
    • PECA1_HUMAN antibody
    • Pecam 1 antibody
    • PECAM 1 CD31 EndoCAM antibody
    • PECAM antibody
    • PECAM-1 antibody
    • Pecam1 antibody
    • Platelet endothelial cell adhesion molecule antibody
    • Platelet/endothelial cell adhesion molecule 1 antibody
    see all

Anti-CD31 antibody [WM59] images

  • FACS profile of endothelial cells stained with anti-CD31 antibody. Solid profiles represent IgG isotype control.
  • ab218 staining CD31 in human peripheral blood lymphocytes by Flow Cytometry analysis.

References for Anti-CD31 antibody [WM59] (ab218)

This product has been referenced in:
  • Abdanipour A  et al. Intraspinal transplantation of motoneuron-like cell combined with delivery of polymer-based glial cell line-derived neurotrophic factor for repair of spinal cord contusion injury. Neural Regen Res 9:1003-13 (2014). Read more (PubMed: 25206752) »
  • Bagley RG  et al. Human endothelial precursor cells express tumor endothelial marker 1/endosialin/CD248. Mol Cancer Ther 7:2536-46 (2008). Flow Cyt ; Human . Read more (PubMed: 18723498) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HT1080 cells)
Specification HT1080 cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
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Verified customer

Submitted Jan 26 2010

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application (IHC-P). All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, you may wish to partic...

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Sorry, cross-reactivity of this antibody with other species is not yet known and it has not been tested in immunohistochemistry.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"