Overview

  • Product nameCD31 (PECAM-1) Monkey ELISA Kit
    See all CD31 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 2%
    Overall 2%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.06 U/ml
  • Range
    0.48 U/ml - 30 U/ml
  • Recovery

    110 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Monkey
  • Product overview

    Abcam’s CD31 (PECAM-1) Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey CD31 concentrations in cell culture supernatant and serum (baboon).

    CD31 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with an biotinylated-CD31 detection antibody. The microplate is then incubated at room temperature. Unbound conjugates are then washed away and TMB is added and catalyzed by HRP to produce a blue color product that subsequently changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the CD31 amount of sample captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionInduces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • Tissue specificityExpressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • Sequence similaritiesContains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainThe Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • Post-translational
    modifications
    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • Cellular localizationMembrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • Alternative names
    • CD31
    • CD31 antigen
    • EndoCAM
    • GPIIA
    • GPIIA''
    • HEC7
    • Leukocyte surface protein
    • PECA1
    • PECA1_HUMAN
    • PECAM 1
    • PECAM-1
    • Pecam1
    • Platelet endothelial cell adhesion molecule
    • Platelet/endothelial cell adhesion molecule
    see all

Applications

Our Abpromise guarantee covers the use of ab119552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

CD31 (PECAM-1) Monkey ELISA Kit images

  • Representative Standard Curve using ab119552.

Protocols

References for CD31 (PECAM-1) Monkey ELISA Kit (ab119552)

ab119552 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119552.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"