Overview

  • Product nameAnti-CD32 antibody [9C6]
    See all CD32 primary antibodies
  • Description
    Mouse monoclonal [9C6] to CD32
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human CD32 produced in HEK293T cells (NP_067674).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY CD32; Human Breast, Colon, Kidney, Lung, Ovary, Ovary adenocarcinoma, Pancreas, Thyroid, Thyroid carcinoma, Endometrium and Prostate tissues; COS7 cells transiently transfected with pCMV6-ENTRY CD32;
  • General notesDilute in PBS (pH7.3) before use. Avoid repeated freeze-thaws.

Properties

Applications

Our Abpromise guarantee covers the use of ab124408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 35 kDa.
IHC-P 1/50.
ICC/IF 1/100.
IP Use a concentration of 4 µg/ml.

Target

  • FunctionBinds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.
  • Tissue specificityFound on monocytes, neutrophils and eosinophil platelets.
  • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD32 antibody
    • CD32A antibody
    • CD32B antibody
    • CD32C antibody
    • CDw32 antibody
    • Fc fragment of IgG low affinity IIa receptor antibody
    • Fc fragment of IgG low affinity IIa receptor for (CD32) antibody
    • Fc fragment of IgG low affinity IIb receptor antibody
    • Fc fragment of IgG low affinity IIb receptor for (CD32) antibody
    • Fc fragment of IgG low affinity IIc receptor antibody
    • Fc fragment of IgG low affinity IIc receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity IIa, receptor (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
    • Fc gamma receptor IIB antibody
    • Fc gamma receptor IIC antibody
    • Fc gamma RII a antibody
    • Fc gamma RII b antibody
    • Fc gamma RII c antibody
    • Fc gamma RIIb antibody
    • Fc gamma RIIc antibody
    • Fc receptor, IgG, low affinity IIb antibody
    • Fc-gamma RII-a antibody
    • Fc-gamma RII-b antibody
    • Fc-gamma RII-c antibody
    • Fc-gamma-RIIa antibody
    • FC-gamma-RIIB antibody
    • Fc-GAMMA-RIIC antibody
    • Fc[g]RII antibody
    • FCG2 antibody
    • FCG2A_HUMAN antibody
    • FcGR antibody
    • FCGR2 antibody
    • FCGR2A antibody
    • FCGR2A1 antibody
    • FCGR2B antibody
    • FCGR2C antibody
    • Fcgr3 antibody
    • Fcgr3a antibody
    • FcgRII antibody
    • Fcr-2 antibody
    • Fcr-3 antibody
    • FcRII a antibody
    • FCRII antibody
    • FcRII b antibody
    • FcRII c antibody
    • FcRII-a antibody
    • FcRIIC antibody
    • IGFR2 antibody
    • IgG Fc receptor II a antibody
    • IgG Fc receptor II b antibody
    • IgG Fc receptor II beta antibody
    • IgG Fc receptor II c antibody
    • IgG Fc receptor II-a antibody
    • IgG Fc receptor II-b antibody
    • IgG Fc receptor II-c antibody
    • Immunoglobulin G Fc receptor II antibody
    • Immunoglobulin G Fc receptor II c antibody
    • Low affinity immunoglobulin gamma Fc region receptor II antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-c antibody
    • Ly-17 antibody
    • Ly-m20 antibody
    • LyM-1 antibody
    • Lymphocyte antigen 17 antibody
    see all

Anti-CD32 antibody [9C6] images

  • All lanes : Anti-CD32 antibody [9C6] (ab124408) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CD32

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 35 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205503)
  • Immunoprecipitation of CD32. Lane 1: Negative control, no IgG. Lane2: 2µg of ab124408 and 20µl (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed with lysate and incubated overnight. For each experiment, 500µl of DDK tagged CD32 overexpression lysates (at 1:5 dilution with HEK293T lysate) was used. Predicted band size : 35 kDa
  • ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human breast tissue by Immunohistochemistry.
  • ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human colon tissue by Immunohistochemistry.
  • ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human lung tissue by Immunohistochemistry.
  • ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human ovary tissue by Immunohistochemistry.
  • ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
  • ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
  • ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human thyroid tissue by Immunohistochemistry.
  • ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
  • ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
  • ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • ab124408, at 1/100 dilution, staining CD32 in COS7 cells transiently transfected with pCMV6-ENTRY CD32 by Immunofluorescence.

References for Anti-CD32 antibody [9C6] (ab124408)

ab124408 has not yet been referenced specifically in any publications.

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