Overview

  • Product name
    Anti-CD32 antibody [AT10] (Biotin)
    See all CD32 primary antibodies
  • Description
    Mouse monoclonal [AT10] to CD32 (Biotin)
  • Conjugation
    Biotin
  • Specificity
    According to the CD32 Cluster workshop (Leucocyte Typing V, pp823, pp828), clone AT10 reacts equally well with both Fc gamma RII a and b-expressing cells.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Dog, Human, Rhesus monkey
  • Immunogen

    Human K562 cell line

  • Positive control
    • Human peripheral blood monocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab30355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 10µl for 106 cells, or 100µl whole blood.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.
    • Tissue specificity
      Found on monocytes, neutrophils and eosinophil platelets.
    • Sequence similarities
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD32 antibody
      • CD32A antibody
      • CD32B antibody
      • CD32C antibody
      • CDw32 antibody
      • Fc fragment of IgG low affinity IIa receptor antibody
      • Fc fragment of IgG low affinity IIa receptor for (CD32) antibody
      • Fc fragment of IgG low affinity IIb receptor antibody
      • Fc fragment of IgG low affinity IIb receptor for (CD32) antibody
      • Fc fragment of IgG low affinity IIc receptor antibody
      • Fc fragment of IgG low affinity IIc receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity IIa, receptor (CD32) antibody
      • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
      • Fc gamma receptor IIB antibody
      • Fc gamma receptor IIC antibody
      • Fc gamma RII a antibody
      • Fc gamma RII b antibody
      • Fc gamma RII c antibody
      • Fc gamma RIIb antibody
      • Fc gamma RIIc antibody
      • Fc receptor, IgG, low affinity IIb antibody
      • Fc-gamma RII-a antibody
      • Fc-gamma RII-b antibody
      • Fc-gamma RII-c antibody
      • Fc-gamma-RIIa antibody
      • FC-gamma-RIIB antibody
      • Fc-GAMMA-RIIC antibody
      • Fc[g]RII antibody
      • FCG2 antibody
      • FCG2A_HUMAN antibody
      • FcGR antibody
      • FCGR2 antibody
      • FCGR2A antibody
      • FCGR2A1 antibody
      • FCGR2B antibody
      • FCGR2C antibody
      • Fcgr3 antibody
      • Fcgr3a antibody
      • FcgRII antibody
      • Fcr-2 antibody
      • Fcr-3 antibody
      • FcRII a antibody
      • FCRII antibody
      • FcRII b antibody
      • FcRII c antibody
      • FcRII-a antibody
      • FcRIIC antibody
      • IGFR2 antibody
      • IgG Fc receptor II a antibody
      • IgG Fc receptor II b antibody
      • IgG Fc receptor II beta antibody
      • IgG Fc receptor II c antibody
      • IgG Fc receptor II-a antibody
      • IgG Fc receptor II-b antibody
      • IgG Fc receptor II-c antibody
      • Immunoglobulin G Fc receptor II antibody
      • Immunoglobulin G Fc receptor II c antibody
      • Low affinity immunoglobulin gamma Fc region receptor II antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-c antibody
      • Ly-17 antibody
      • Ly-m20 antibody
      • LyM-1 antibody
      • Lymphocyte antigen 17 antibody
      see all

    Images

    • Flow cytometric analysis of human peripheral blood monocytes with 10µl for 106 cells of anti human CD32 Biotin conjugated (ab30355).

    References

    This product has been referenced in:
    • Lilliehöök I  et al. Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies. Vet Immunol Immunopathol 61:181-93 (1998). Read more (PubMed: 9613433) »
    • Metes D  et al. Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene. Blood 91:2369-80 (1998). Read more (PubMed: 9516136) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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