Recombinant Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6657(2)] to CD32B + CD32A + CD32C
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] -
Description
Rabbit monoclonal [EPR6657(2)] to CD32B + CD32A + CD32C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD32A. The exact sequence is proprietary.
(Peptide available asab211774) -
Positive control
- WB: K562, U937, Daudi, human placenta and human platelet lysates. IHC-P: Human stomach and tonsil tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6657(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 37-42 kDa (predicted molecular weight: 35 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified format use at 1/250 - 1/500 dilution. |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 37-42 kDa (predicted molecular weight: 35 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified format use at 1/250 - 1/500 dilution. |
Target
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Cellular localization
CD32B: Cell membrane. CD32A: Cell membrane. CD32C: Cytoplasm and Cell membrane. -
Database links
- Entrez Gene: 2212 Human
- Entrez Gene: 2213 Human
- Entrez Gene: 9103 Human
- Omim: 146790 Human
- Omim: 604590 Human
- SwissProt: P12318 Human
- SwissProt: P31994 Human
- SwissProt: P31995 Human
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD32A + CD32B + CD32C with purified ab155972 at 1/1000 dilution (0.98 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972) at 1/10000 dilution (Purified) + U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 37-42 kDa why is the actual band size different from the predicted? -
All lanes : Anti-CD32B + CD32A + CD32C antibody [EPR6657(2)] (ab155972) at 1/1000 dilution (unpurified)
Lane 1 : K562 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : Human placenta lysate
Lane 5 : Human platelet lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 37-42 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue labeling CD32A + CD32B + CD32C with ab155972 (unpurified) at 1/250 dilution
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ab155972 (unpurified) showing -ve staining in Human skeletal muscle.
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ab155972 (unpurified) showing +ve staining in Human placenta.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD32A + CD32B + CD32C with ab155972 (unpurified) at 1/250 dilution
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab155972 has been referenced in 3 publications.
- Swanson MEV et al. Quantitative immunohistochemical analysis of myeloid cell marker expression in human cortex captures microglia heterogeneity with anatomical context. Sci Rep 10:11693 (2020). PubMed: 32678124
- Cao J et al. Versatile and on-demand biologics co-production in yeast. Nat Commun 9:77 (2018). WB . PubMed: 29311542
- Sips M et al. Fc receptor-mediated phagocytosis in tissues as a potent mechanism for preventive and therapeutic HIV vaccine strategies. Mucosal Immunol 9:1584-1595 (2016). IHC ; Human . PubMed: 26883728