Overview

  • Product name
    Anti-CD32 antibody [EPR6658]
    See all CD32 primary antibodies
  • Description
    Rabbit monoclonal [EPR6658] to CD32
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    A synthetic peptide, corresponding to residues in Human CD32.

  • Positive control
    • K562 and Human platelet lysates; Human lung and spleen tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab125013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 37-43 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.
    • Tissue specificity
      Found on monocytes, neutrophils and eosinophil platelets.
    • Sequence similarities
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD32 antibody
      • CD32A antibody
      • CD32B antibody
      • CD32C antibody
      • CDw32 antibody
      • Fc fragment of IgG low affinity IIa receptor antibody
      • Fc fragment of IgG low affinity IIa receptor for (CD32) antibody
      • Fc fragment of IgG low affinity IIb receptor antibody
      • Fc fragment of IgG low affinity IIb receptor for (CD32) antibody
      • Fc fragment of IgG low affinity IIc receptor antibody
      • Fc fragment of IgG low affinity IIc receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity IIa, receptor (CD32) antibody
      • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
      • Fc gamma receptor IIB antibody
      • Fc gamma receptor IIC antibody
      • Fc gamma RII a antibody
      • Fc gamma RII b antibody
      • Fc gamma RII c antibody
      • Fc gamma RIIb antibody
      • Fc gamma RIIc antibody
      • Fc receptor, IgG, low affinity IIb antibody
      • Fc-gamma RII-a antibody
      • Fc-gamma RII-b antibody
      • Fc-gamma RII-c antibody
      • Fc-gamma-RIIa antibody
      • FC-gamma-RIIB antibody
      • Fc-GAMMA-RIIC antibody
      • Fc[g]RII antibody
      • FCG2 antibody
      • FCG2A_HUMAN antibody
      • FcGR antibody
      • FCGR2 antibody
      • FCGR2A antibody
      • FCGR2A1 antibody
      • FCGR2B antibody
      • FCGR2C antibody
      • Fcgr3 antibody
      • Fcgr3a antibody
      • FcgRII antibody
      • Fcr-2 antibody
      • Fcr-3 antibody
      • FcRII a antibody
      • FCRII antibody
      • FcRII b antibody
      • FcRII c antibody
      • FcRII-a antibody
      • FcRIIC antibody
      • IGFR2 antibody
      • IgG Fc receptor II a antibody
      • IgG Fc receptor II b antibody
      • IgG Fc receptor II beta antibody
      • IgG Fc receptor II c antibody
      • IgG Fc receptor II-a antibody
      • IgG Fc receptor II-b antibody
      • IgG Fc receptor II-c antibody
      • Immunoglobulin G Fc receptor II antibody
      • Immunoglobulin G Fc receptor II c antibody
      • Low affinity immunoglobulin gamma Fc region receptor II antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-c antibody
      • Ly-17 antibody
      • Ly-m20 antibody
      • LyM-1 antibody
      • Lymphocyte antigen 17 antibody
      see all

    Images

    • All lanes : Anti-CD32 antibody [EPR6658] (ab125013) at 1/1000 dilution

      Lane 1 : K562 lysate
      Lane 2 : Human platelet lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 35 kDa
      Observed band size : 37-43 kDa (why is the actual band size different from the predicted?)
    • ab125013 at 1/250 staining CD32 in paraffin-embedded Human lung tissue by immunohistochemistry.
    • ab125013 at 1/250 staining CD32 in paraffin-embedded Human spleen tissue by immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Murinello S  et al. Fc? receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration. Invest Ophthalmol Vis Sci 55:247-58 (2014). IHC-P ; Human . Read more (PubMed: 24334446) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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