Anti-CD32A antibody [OTI9C6] (ab124408)
Key features and details
- Mouse monoclonal [OTI9C6] to CD32A
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-CD32A antibody [OTI9C6]
See all CD32A primary antibodies -
Description
Mouse monoclonal [OTI9C6] to CD32A -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human CD32A.
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Positive control
- HEK293T cell lysate transfected with pCMV6-ENTRY CD32; Human Breast, Colon, Kidney, Lung, Ovary, Ovary adenocarcinoma, Pancreas, Thyroid, Thyroid carcinoma, Endometrium and Prostate tissues; COS7 cells transiently transfected with pCMV6-ENTRY CD32;
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General notes
Dilute in PBS (pH7.3) before use. Avoid repeated freeze-thaws.
The clone number has been updated from 9C6 to OTI9C6, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI9C6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 35 kDa.
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IHC-P |
1/50.
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ICC/IF |
1/100.
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IP |
Use a concentration of 4 µg/ml.
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Notes |
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WB
1/2000. Predicted molecular weight: 35 kDa. |
IHC-P
1/50. |
ICC/IF
1/100. |
IP
Use a concentration of 4 µg/ml. |
Target
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Function
Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. -
Tissue specificity
Found on monocytes, neutrophils and eosinophil platelets. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2212 Human
- Omim: 146790 Human
- SwissProt: P12318 Human
- Unigene: 352642 Human
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Alternative names
- CD32 antibody
- CD32 antigen antibody
- CDw32 antibody
see all
Images
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ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human breast tissue by Immunohistochemistry.
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All lanes : Anti-CD32A antibody [OTI9C6] (ab124408) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CD32
Lysates/proteins at 5 µg per lane.
Predicted band size: 35 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205503) -
Immunoprecipitation of CD32A. Lane 1: Negative control, no IgG. Lane2: 2µg of ab124408 and 20µl (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed with lysate and incubated overnight. For each experiment, 500µl of DDK tagged CD32 overexpression lysates (at 1:5 dilution with HEK293T lysate) was used. Predicted band size : 35 kDa
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ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human colon tissue by Immunohistochemistry.
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ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human kidney tissue by Immunohistochemistry.
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ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human lung tissue by Immunohistochemistry.
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ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human ovary tissue by Immunohistochemistry.
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ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
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ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
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ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human thyroid tissue by Immunohistochemistry.
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ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
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ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
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ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human prostate tissue by Immunohistochemistry.
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ab124408, at 1/100 dilution, staining CD32A in COS7 cells transiently transfected with pCMV6-ENTRY CD32 by Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab124408 has not yet been referenced specifically in any publications.