Anti-CD32 (phospho Y292) antibody [EP926Y] (ab68423)

Overview

  • Product nameAnti-CD32 (phospho Y292) antibody [EP926Y]
    See all CD32 primary antibodies
  • Description
    Rabbit monoclonal [EP926Y] to CD32 (phospho Y292)
  • SpecificityDetects CD32b only when phosphorylated on Tyrosine 292.
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding tyrosine 292 of Human CD32

  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab68423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/100000. Detects a band of approximately 40,44 kDa (predicted molecular weight: 40,44 kDa).
IP 1/70.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • FunctionBinds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.
    • Tissue specificityFound on monocytes, neutrophils and eosinophil platelets.
    • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD32 antibody
      • CD32A antibody
      • CD32B antibody
      • CD32C antibody
      • CDw32 antibody
      • Fc fragment of IgG low affinity IIa receptor antibody
      • Fc fragment of IgG low affinity IIa receptor for (CD32) antibody
      • Fc fragment of IgG low affinity IIb receptor antibody
      • Fc fragment of IgG low affinity IIb receptor for (CD32) antibody
      • Fc fragment of IgG low affinity IIc receptor antibody
      • Fc fragment of IgG low affinity IIc receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity IIa, receptor (CD32) antibody
      • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
      • Fc gamma receptor IIB antibody
      • Fc gamma receptor IIC antibody
      • Fc gamma RII a antibody
      • Fc gamma RII b antibody
      • Fc gamma RII c antibody
      • Fc gamma RIIb antibody
      • Fc gamma RIIc antibody
      • Fc receptor, IgG, low affinity IIb antibody
      • Fc-gamma RII-a antibody
      • Fc-gamma RII-b antibody
      • Fc-gamma RII-c antibody
      • Fc-gamma-RIIa antibody
      • FC-gamma-RIIB antibody
      • Fc-GAMMA-RIIC antibody
      • Fc[g]RII antibody
      • FCG2 antibody
      • FCG2A_HUMAN antibody
      • FcGR antibody
      • FCGR2 antibody
      • FCGR2A antibody
      • FCGR2A1 antibody
      • FCGR2B antibody
      • FCGR2C antibody
      • Fcgr3 antibody
      • Fcgr3a antibody
      • FcgRII antibody
      • Fcr-2 antibody
      • Fcr-3 antibody
      • FcRII a antibody
      • FCRII antibody
      • FcRII b antibody
      • FcRII c antibody
      • FcRII-a antibody
      • FcRIIC antibody
      • IGFR2 antibody
      • IgG Fc receptor II a antibody
      • IgG Fc receptor II b antibody
      • IgG Fc receptor II beta antibody
      • IgG Fc receptor II c antibody
      • IgG Fc receptor II-a antibody
      • IgG Fc receptor II-b antibody
      • IgG Fc receptor II-c antibody
      • Immunoglobulin G Fc receptor II antibody
      • Immunoglobulin G Fc receptor II c antibody
      • Low affinity immunoglobulin gamma Fc region receptor II antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-c antibody
      • Ly-17 antibody
      • Ly-m20 antibody
      • LyM-1 antibody
      • Lymphocyte antigen 17 antibody
      see all

    Anti-CD32 (phospho Y292) antibody [EP926Y] images

    • All lanes : Anti-CD32 (phospho Y292) antibody [EP926Y] (ab68423) at 1/100000 dilution

      Lane 1 : Untreated Daudi cell lysate
      Lane 2 : Pervanadate-treated Daudi cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

      Predicted band size : 40,44 kDa

    References for Anti-CD32 (phospho Y292) antibody [EP926Y] (ab68423)

    This product has been referenced in:
    • Rieth N  et al. Characterization of SM201, an anti-hFc?RIIB antibody not interfering with ligand binding that mediates immune complex dependent inhibition of B cells. Immunol Lett 160:145-50 (2014). Read more (PubMed: 24472604) »
    • Sundgren NC  et al. Coupling of fc? receptor I to fc? receptor IIb by SRC kinase mediates C-reactive protein impairment of endothelial function. Circ Res 109:1132-40 (2011). Read more (PubMed: 21940940) »

    See all 3 Publications for this product

    Product Wall

    Vielen Dank für Ihren Anruf.

    Ich kann Ihnen nun bestätigen, dass beide Antikörper spezifisch die IIb Isoform (und alle Unterformen von dieser Isoform: IIb1, IIb2, IIb3) binden.

    Beide Immunogen sind Peptide die die fol...

    Read More

    ab68423 detects CD32b and is not expected to cross-react with CD32a. However, this has not been specifically tested in the laboratory as far as I am aware.

    Thank you very much for contacting us with your question.

    I apologize for the confusing discrepancy on the Abcam and Epitomics datasheets. I've gotten in touch with the Epitomics lab, and they did test the antibody once on mouse samples (brai...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"