Overview

  • Product name
  • Description
    Rabbit polyclonal to CD320
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    fragment corresponding to C terminal amino acids 255-282 (AQERLRPLGLLVAMKESLLLSEQKTSLP) of Human CD320.

  • Positive control
    • Human lymph node tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin.
  • Tissue specificity
    Expressed abundantly on follicular dendritic cells (FDCs).
  • Involvement in disease
    Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC) [MIM:613646]; also called methylmalonic aciduria due to transcobalamin receptor defect. MMATC is a metabolic disorder characterized by increased blood C3-acylcarnitine levels, elevated methylmalonate and homocysteine, and low uptake of transcobalamin-bound cobalamin, but normal conversion to adenosylcobalamin and methylcobalamin. Plasma vitamin B12 and total homocysteine are normal.
  • Sequence similarities
    Contains 2 LDL-receptor class A domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 8D6 antibody
    • 8D6 antigen antibody
    • 8D6A antibody
    • CD320 antibody
    • CD320 antigen antibody
    • CD320_HUMAN antibody
    • FDC-signaling molecule 8D6 antibody
    • FDC-SM-8D6 antibody
    • TCblR antibody
    • Transcobalamin receptor antibody
    see all

Images

  • ab121433 at a 1/50 dilution staining CD320 in paraffin-embedded Human lymph node tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Fowler R  et al. Uptake and transport of B12-conjugated nanoparticles in airway epithelium. J Control Release 172:374-81 (2013). ICC/IF ; Human . Read more (PubMed: 24008152) »

See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Human Purified protein (Hep G2 (Liver))
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (Stacking gel (10%), Resolving gel (10%))
Loading amount
20 µg
Treatment
10 concentrations of Vitamin B12 (500nM, 400nM, 200nM, 100nM, 50nM, 20nM, 10nM, 1nM, 100pM and 25pM) for 10 days
Specification
Hep G2 (Liver)
Blocking step
(agent) for 12 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 4°C
Username

Joseph Boachie

Verified customer

Submitted Aug 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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