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Synthetic peptide corresponding to Human CD32B (C terminal).
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab45143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 49-55 kDa.|
|IHC-P||1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).
Unpurified ab45143 staining human CD32 (1/100) in human lymph node by immunohistochemistry using paraffin embedded tissue.
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Exposure times: Lanes 1 and 2: 3 minutes, Lanes 3 and 4: 15 seconds, Lanes 5 and 6: 3 minutes.
Blocking and dilution buffer: 5% NFDM/TBST.