Anti-CD32B (phospho Y292) antibody (ab74092)

Overview

  • Product nameAnti-CD32B (phospho Y292) antibody
    See all CD32B primary antibodies
  • Description
    Rabbit polyclonal to CD32B (phospho Y292)
  • Specificityab74092 detects endogenous levels of CD32 only when phosphorylated at tyrosine 292.
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from human CD32B around the phosphorylation site of tyrosine 292 (I-T-YP-S-L). (SwissProt P31994).

  • Positive control
    • K562 cell extracts treated with PMA (125ng/ml, 30mins). IHC-P (FFPE): Human Placenta (Normal)

Properties

Applications

Our Abpromise guarantee covers the use of ab74092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
ELISA 1/1000.

Target

  • FunctionReceptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.
  • Tissue specificityIs the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.
  • Involvement in diseaseNote=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression.
  • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainContains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD32 antibody
    • CD32 antigen antibody
    • CD32B antibody
    • CDw32 antibody
    • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor antibody
    • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor for antibody
    • Fc gamma receptor IIB antibody
    • Fc gamma RII antibody
    • Fc gamma RIIB antibody
    • Fc-gamma RII antibody
    • Fc-gamma RII-b antibody
    • Fc-gamma-RIIb antibody
    • FCG2 antibody
    • FCG2B_HUMAN antibody
    • Fcgr2 antibody
    • FCGR2B antibody
    • FCRII antibody
    • FcRII-b antibody
    • IGFR2 antibody
    • IgG Fc receptor II beta antibody
    • IgG Fc receptor II-b antibody
    • Low affinity immunoglobulin gamma Fc region receptor II antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
    • Ly-17 antibody
    • Lym 1 antibody
    • Lymphocyte antigen 17 antibody
    see all

Anti-CD32B (phospho Y292) antibody images

  • All lanes : Anti-CD32B (phospho Y292) antibody (ab74092) at 1/500 dilution

    Lane 1 : K562 cell extracts treated with PMA (125ng/ml, 30mins)
    Lane 2 : K562 cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • IHC image of CD32B staining in Human Placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74092, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-CD32B (phospho Y292) antibody (ab74092)

ab74092 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (purified splenic B cells)
Gel Running Conditions Reduced Denaturing
Loading amount 35 µg
Treatment 10ug/ml anti-FcgRIIb for 15 min., 20ugml anti-IgM (whole molecule) 15 min.
Specification purified splenic B cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Sep 30 2015

Vielen Dank für Ihren Anruf.

Ich kann Ihnen nun bestätigen, dass beide Antikörper spezifisch die IIb Isoform (und alle Unterformen von dieser Isoform: IIb1, IIb2, IIb3) binden.

Beide Immunogen sind Peptide die die fol...

Read More

We have three CD32B antibodies, I compared their immunogen sequences against the CD32A. Results are following: ab87965- Mouse monoclonal to CD32B homology with CD32A is 92%. Cross-reactivity with CD32A has not been tested. It is likely that there will...

Read More

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