Overview

  • Product name
    Anti-CD33 antibody [6C5/2]
    See all CD33 primary antibodies
  • Description
    Mouse monoclonal [6C5/2] to CD33
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    CD33-Fc recombinant protein.

  • Positive control
    • IHC-P: Human tonsil FFPE tissue sections

Properties

Applications

Our Abpromise guarantee covers the use of ab11032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
  • Tissue specificity
    Monocytic/myeloid lineage cells.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Domain
    Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Post-translational
    modifications
    Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 33 antibody
    • CD33 antibody
    • CD33 antigen (gp67) antibody
    • CD33 antigen antibody
    • CD33 molecule antibody
    • CD33_HUMAN antibody
    • FLJ00391 antibody
    • gp67 antibody
    • My9 antibody
    • Myeloid cell surface antigen CD33 antibody
    • Myeloid cell surface antigen CD33 precursor antibody
    • Myeloid differentiation antigen CD33 antibody
    • p67 antibody
    • Sialic acid binding Ig like lectin 3 antibody
    • Sialic acid binding immunoglobulin like lectin 3 antibody
    • Sialic acid-binding Ig-like lectin 3 antibody
    • SIGLEC 3 antibody
    • Siglec-3 antibody
    • SIGLEC3 antibody
    see all

Anti-CD33 antibody [6C5/2] images

  • Flow Cytometry analysis of untransfected (open histogram) or Siglec-transfected CHO cells (grey histogram) were incubated with gp120 after being blocked with BSA and murine IgG, and then stained with PE-conjugated streptavidin.

  • IHC image of CD33 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11032, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Flow Cytometry analysis of untransfected (open histogram) or Siglec-transfected CHO cells (grey histogram), pretreated with neuraminidase (NA) were incubated with gp120 after being blocked with BSA and murine IgG, and then stained with PE-conjugated streptavidin.

References for Anti-CD33 antibody [6C5/2] (ab11032)

This product has been referenced in:
  • Zou Z  et al. Siglecs Facilitate HIV-1 Infection of Macrophages through Adhesion with Viral Sialic Acids. PLoS One 6:e24559 (2011). Flow Cyt . Read more (PubMed: 21931755) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin melanoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH
Permeabilization
No
Specification
Skin melanoma
Blocking step
Hydrogen peroxide as blocking agent for 5 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 02 2015

Thank you very much for your call yesterday and for your patience while I have been in touch with the lab regarding this antibody.

The lab scientists confirmed that the antibody cross-reacts with mouse samples, but unfortunately we don't have...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up