• Product nameAnti-CD33 antibody [WM53]
    See all CD33 primary antibodies
  • Description
    Mouse monoclonal [WM53] to CD33
  • Tested applicationsSuitable for: WB, IP, IHC-Fr, Functional Studies, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Non Human Primates
  • Immunogen

    Human AML cells.



Our Abpromise guarantee covers the use of ab119860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. (acetone fixation).
Functional Studies Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionPutative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
  • Tissue specificityMonocytic/myeloid lineage cells.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • DomainContains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Post-translational
    Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 33 antibody
    • CD33 antibody
    • CD33 antigen (gp67) antibody
    • CD33 antigen antibody
    • CD33 molecule antibody
    • CD33_HUMAN antibody
    • FLJ00391 antibody
    • gp67 antibody
    • My9 antibody
    • Myeloid cell surface antigen CD33 antibody
    • Myeloid cell surface antigen CD33 precursor antibody
    • Myeloid differentiation antigen CD33 antibody
    • p67 antibody
    • Sialic acid binding Ig like lectin 3 antibody
    • Sialic acid binding immunoglobulin like lectin 3 antibody
    • Sialic acid-binding Ig-like lectin 3 antibody
    • SIGLEC 3 antibody
    • Siglec-3 antibody
    • SIGLEC3 antibody
    see all

References for Anti-CD33 antibody [WM53] (ab119860)

This product has been referenced in:
  • Yi L  et al. Involvement of calreticulin in cell proliferation, invasion and differentiation in diallyl disulfide-treated HL-60 cells. Oncol Lett 12:1861-1867 (2016). Human . Read more (PubMed: 27588133) »
  • Hernández-López C  et al. Stromal cell-derived factor 1/CXCR4 signaling is critical for early human T-cell development. Blood 99:546-54 (2002). Read more (PubMed: 11781237) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Human Cell (Human bone marrow smear)
Specification Human bone marrow smear
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Fixative Paraformaldehyde

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 27 2013

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

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