• Product nameAnti-CD33 antibody [WM53]
    See all CD33 primary antibodies
  • Description
    Mouse monoclonal [WM53] to CD33
  • Tested applicationsSuitable for: ICC, Flow Cyt, IP, WB, IHC-Frmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human AML cells.

  • Positive control
    • Lymphatic tissue



Our Abpromise guarantee covers the use of ab30371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/50.
Flow Cyt 1/10 - 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. May also detects the smaller precursor protein at 40 kDa.
IHC-Fr 1/50 - 1/100.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionPutative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
    • Tissue specificityMonocytic/myeloid lineage cells.
    • Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • DomainContains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Post-translational
      Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 33 antibody
      • CD33 antibody
      • CD33 antigen (gp67) antibody
      • CD33 antigen antibody
      • CD33 molecule antibody
      • CD33_HUMAN antibody
      • FLJ00391 antibody
      • gp67 antibody
      • My9 antibody
      • Myeloid cell surface antigen CD33 antibody
      • Myeloid cell surface antigen CD33 precursor antibody
      • Myeloid differentiation antigen CD33 antibody
      • p67 antibody
      • Sialic acid binding Ig like lectin 3 antibody
      • Sialic acid binding immunoglobulin like lectin 3 antibody
      • Sialic acid-binding Ig-like lectin 3 antibody
      • SIGLEC 3 antibody
      • Siglec-3 antibody
      • SIGLEC3 antibody
      see all

    Anti-CD33 antibody [WM53] images

    • ab30371 staining human peripheral blood monocytes by flow cytometry

    References for Anti-CD33 antibody [WM53] (ab30371)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cell (Cytospin (human leukemia cells))
    Specification Cytospin (human leukemia cells)
    Fixative Acetone
    Permeabilization No

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    Submitted May 22 2009