The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells.
ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Belongs to the CD34 family.
On early hematopoietic progenitor cells.
Highly glycosylated. Phosphorylated on serine residues by PKC.
Flow Cytometric analysis of normal Human peripheral blood cells labeling CD34 with Anti-Human CD45 FITC and either ab155377 (right histogram) at 0.25 µg/test in 100 µl final volume or an equal amount of Mouse IgG1 kappa Isotype Control APC (left histogram). Cells in the lymphocyte gate were used for analysis.