Overview

  • Product nameAnti-CD34 antibody [4H11(APG)]
    See all CD34 primary antibodies
  • Description
    Mouse monoclonal [4H11(APG)] to CD34
  • SpecificityThis antibody reacts with Class III epitope on CD34 antigen.
  • Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human). (Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia).

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 2 µg/ml. Use under non reducing condition. Predicted molecular weight: 115 kDa.
Flow Cyt Use a concentration of 2 µg/ml.

Target

  • FunctionPossible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • Tissue specificitySelectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • Sequence similaritiesBelongs to the CD34 family.
  • Developmental stageOn early hematopoietic progenitor cells.
  • Post-translational
    modifications
    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

Anti-CD34 antibody [4H11(APG)] images

  • IHC image of CD34 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab762, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-CD34 antibody [4H11(APG)] (ab762)

This product has been referenced in:

See 1 Publication for this product

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