Overview

  • Product nameAnti-CD34 antibody [4H11[APG]] (FITC)
    See all CD34 primary antibodies
  • Description
    Mouse monoclonal [4H11[APG]] to CD34 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThe antibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.

  • EpitopeClass III epitope on CD34 antigen.
  • Positive control
    • Human blood cells.

Applications

Our Abpromise guarantee covers the use of ab18227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 20 µl reagent / 100 µl sample.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPossible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
    • Tissue specificitySelectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
    • Sequence similaritiesBelongs to the CD34 family.
    • Developmental stageOn early hematopoietic progenitor cells.
    • Post-translational
      modifications
      Highly glycosylated.
      Phosphorylated on serine residues by PKC.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD34 antibody
      • CD34 antigen antibody
      • CD34 molecule antibody
      • CD34_HUMAN antibody
      • Cluster designation 34 antibody
      • Hematopoietic progenitor cell antigen CD34 antibody
      • HPCA1 antibody
      • Mucosialin antibody
      • OTTHUMP00000034733 antibody
      • OTTHUMP00000034734 antibody
      see all

    Anti-CD34 antibody [4H11[APG]] (FITC) images

    • Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC (ab18227). Total viable cells were used for analysis.

      ab18227 was used at a recommended dilution of 2 μg/ml.

    References for Anti-CD34 antibody [4H11[APG]] (FITC) (ab18227)

    This product has been referenced in:
    • Wu YT  et al. A novel and feasible way to cultivate and purify endothelial progenitor cells from bone marrow of children with congenital heart diseases. Chin Med J (Engl) 125:1903-7 (2012). Flow Cyt ; Human . Read more (PubMed: 22884051) »
    • Sampat SR  et al. Growth factor priming of synovium-derived stem cells for cartilage tissue engineering. Tissue Eng Part A 17:2259-65 (2011). Flow Cyt ; Cow . Read more (PubMed: 21542714) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting us. The concentration of ab18227, anti-CD34, has been determined and is 50 ug/ml for the lot that you have. So, 20 ul contains 1 ug.

    Abcam also carries other products essential for Flow Cytometry such as isotype cont...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"