Anti-CD34 antibody [4H11(APG)], prediluted (Phycoerythrin) (ab18228)

Overview

  • Product nameAnti-CD34 antibody [4H11(APG)], prediluted (Phycoerythrin)
    See all CD34 primary antibodies
  • Description
    Mouse monoclonal [4H11(APG)] to CD34, prediluted (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityThe antibody reacts with Class III epitope on CD34 antibody. It completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia).

  • Positive control
    • CML cell line MOLM-7.

Properties

Applications

Our Abpromise guarantee covers the use of ab18228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 20µl for 106 cells. A cell suspension volume of 100 µl is recommended.



ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionPossible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • Tissue specificitySelectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • Sequence similaritiesBelongs to the CD34 family.
  • Developmental stageOn early hematopoietic progenitor cells.
  • Post-translational
    modifications
    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

Anti-CD34 antibody [4H11(APG)], prediluted (Phycoerythrin) images

  • ab18228 staining CD34 in KG-1a cell line by Flow Cytometery. Blue peak repersents negative control while red peak show signal with ab18228.

References for Anti-CD34 antibody [4H11(APG)], prediluted (Phycoerythrin) (ab18228)

This product has been referenced in:
  • Castro FV  et al. 5T4 oncofetal antigen is expressed in high risk of relapse childhood pre-B acute lymphoblastic leukemia and is associated with a more invasive and chemotactic phenotype. Leukemia 26:1487-98 (2012). Flow Cyt ; Human . Read more (PubMed: 22266911) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Fixation NONE
Sample Human Cell (B cell)
Specification B cell
Permeabilization No
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Submitted Feb 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"