The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 20µl for 106 cells. A cell suspension volume of 100 µl is recommended.
ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionPossible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Tissue specificitySelectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Sequence similaritiesBelongs to the CD34 family.
Developmental stageOn early hematopoietic progenitor cells.
Post-translational modificationsHighly glycosylated. Phosphorylated on serine residues by PKC.
ab18228 staining CD34 in KG-1a cell line by Flow Cytometery. Blue peak repersents negative control while red peak show signal with ab18228.
References for Anti-CD34 antibody [4H11(APG)], prediluted (Phycoerythrin) (ab18228)
This product has been referenced in:
Castro FV et al. 5T4 oncofetal antigen is expressed in high risk of relapse childhood pre-B acute lymphoblastic leukemia and is associated with a more invasive and chemotactic phenotype. Leukemia26:1487-98 (2012).
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