The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10µl for 106 cells. ab118658-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionPossible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Tissue specificitySelectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Sequence similaritiesBelongs to the CD34 family.
Developmental stageOn early hematopoietic progenitor cells.
Post-translational modificationsHighly glycosylated. Phosphorylated on serine residues by PKC.
Flow Cytometry analysis of labeling CD34 with Anti-CD34 antibody  (PerCP) (ab119840).
References for Anti-CD34 antibody  (PerCP) (ab119840)
This product has been referenced in:
Ono F et al. CD34+ cells in the peripheral blood transport herpes simplex virus DNA fragments to the skin of patients with erythema multiforme (HAEM). J Invest Dermatol124:1215-24 (2005).
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