The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50000 - 1/200000. Detects a band of approximately 120 kDa (predicted molecular weight: 41 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Belongs to the CD34 family.
On early hematopoietic progenitor cells.
Highly glycosylated. Phosphorylated on serine residues by PKC.