Recombinant
RabMAb

Anti-CD34 antibody [EPR2999] (ab110643)

Overview

  • Product name
    Anti-CD34 antibody [EPR2999]
    See all CD34 primary antibodies
  • Description
    Rabbit monoclonal [EPR2999] to CD34
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human CD34.

  • Positive control
    • Human bone marrow tissue lysate TF-1 cell lysate Human kidney tissue and Human tonsil tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Applications

Our Abpromise guarantee covers the use of ab110643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 120 kDa (predicted molecular weight: 41 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/50.

Target

  • Function
    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • Tissue specificity
    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • Sequence similarities
    Belongs to the CD34 family.
  • Developmental stage
    On early hematopoietic progenitor cells.
  • Post-translational
    modifications
    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cd34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

Anti-CD34 antibody [EPR2999] images

  • All lanes : Anti-CD34 antibody [EPR2999] (ab110643) at 1/50000 dilution

    Lane 1 : Human bone marrow tissue lysate
    Lane 2 : TF-1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size : 41 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
  • ab110643 showing positive staining in Normal stomach vessels tissue.

  • ab110643 showing positive staining in Normal placenta vessels tissue.

  • ab110643 showing positive staining in Normal lung vessels tissue.

  • ab110643 showing positive staining in Normal breast vessels tissue.

  • ab110643, at 1/100, staining CD34 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab110643, at 1/100, staining CD34 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

References for Anti-CD34 antibody [EPR2999] (ab110643)

This product has been referenced in:
  • Wang TB  et al. Sclerosing angiomatoid nodular transformation of the spleen: A case report and literature review. Oncol Lett 12:928-932 (2016). IHC ; Human . Read more (PubMed: 27446372) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako enzyme block and protein block as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH 6
Sample
Human Tissue sections (adipose)
Specification
adipose
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Jean-Martin Lapointe

Verified customer

Submitted Sep 12 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Sample
Human Tissue sections (ovarian cancer)
Specification
ovarian cancer
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. C Ricciardelli

Verified customer

Submitted Aug 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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