Overview

  • Product nameAnti-CD34 antibody [MEC 14.7]See all CD34 primary antibodies ...
  • Description
    Rat monoclonal [MEC 14.7] to CD34
  • Tested applicationsICC/IF, Flow Cyt, IHC-Fr, IP, IHC-P, WB, ELISA, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Murine transformed EC t-end.

  • Positive control
    • Ab8158 gave a positive signal in western blot on mouse lung tissue lysate. This antibody also gave a positive signal in IHC on formalin-fixed, parffin-embedded mouse brain tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab8158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/50.

See Sho et al and Garlanda et al.

IHC-Fr 1/50.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 0.5 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Staining with this method can be difficult, it has been reported to us that milder fixation methods for paraffin sections like using zinc solution work well (unfortunately we have no further detailed instructions of this fixation method).
WB Use a concentration of 5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 41 kDa).
ELISA Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20382702

Target

  • FunctionPossible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • Tissue specificitySelectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • Sequence similaritiesBelongs to the CD34 family.
  • Developmental stageOn early hematopoietic progenitor cells.
  • Post-translational
    modifications
    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD34 antibody
    • Cd34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

Anti-CD34 antibody [MEC 14.7] images

  • Anti-CD34 antibody [MEC 14.7] (ab8158) at 5 µg/ml + Mouse Lung Whole Cell Lysate at 50 µg

    Secondary
    Peroxidase Conjugated Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa


    Exposure time : 20 minutes
  • Rat anti CD34 ab8158 at 2ug/mL (1/50, 1h RT) resulted in specific staining of endothelial cells (mouse xenograft tumor, FFPE, 5 um) with and without Antigen Retrieval (citrate, pH 6).
  • Rat anti CD34 at 2ug/mL (1/50, 1h RT) resulted in specific staining of endothelial cells (mouse xenograft tumor, FFPE, 5 um) with and without Antigen Retrieval (citrate, pH 6).
  • Ab8158 at a dilution of 1/50 staining CD34 from mouse lung cells by Immunocytochemistry. The antibody was incubated with the sample for 1 hour and then detected using an Alexa-Fluor 488® goat anti-rat antibody. The staining for CD34 is shown in green. The samples were also stained for Smooth Muscle Actin (red stain).

    This image is courtesy of an Abreview submitted on 8 February 2006.

    See Abreview

  • ab8158 staining mouse brain tissue sections by IHC-P.  Tissue sections were paraformaldehyde fixed and blocked with 0.5% Perkin-Elmer TNB Blocking Buffer for 30 minutes at 25°C.  The primary antibody was diluted 1/100 and incubated for 18 hours at 4°C.  An biotin conjugated goat anti-rat was used as the secondary

    Photomicrograph demonstrates CD34 in red and collagen type IV (ab19808) in blue in normal, adult brain vessels. Tissue was perfusion-fixed and cut into 15µm slide-mounted cryostat sections (i.e., lightly fixed, but not paraffin embedded).

    See Abreview

  • ab8158 staining CD34 in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 5% BSA for 1 hour at 250C. The sample was incubated with primary antibody (1/100) in PBS for 12 hours at 40C. A Cy3®-conjugated Donkey polyclonal to rat IgG was used as secondary antibody at 1/500 dilution.

    See Abreview

  • IHC image of CD34 staining in Mouse brain FFPE section, performed on a BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8158, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • Immunohistochemical analysis of murine lung tissue, staining CD34 with ab8158.

    Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in diluent) for 16 hours at 4°C. An undiluted HRP-conjugated horse anti-rat polyclonal IgG was used as the secondary antibody.

    See Abreview

References for Anti-CD34 antibody [MEC 14.7] (ab8158)

This product has been referenced in:
  • Li W  et al. GRK3 is essential for metastatic cells and promotes prostate tumor progression. Proc Natl Acad Sci U S A 111:1521-6 (2014). Read more (PubMed: 24434559) »
  • Bohuslavova R  et al. Partial deficiency of HIF-1a stimulates pathological cardiac changes in streptozotocin-induced diabetic mice. BMC Endocr Disord 14:11 (2014). IHC-P ; Mouse . Read more (PubMed: 24502509) »

See all 61 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate (pH 6)
Sample Mouse Tissue sections (Metastatic Lung)
Specification Metastatic Lung
Permeabilization No
Fixative Formaldehyde
Username

Ms. VIctoria Thompson

Verified customer

Submitted Feb 05 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 4°C
Sample Mouse Cell (Lung Endothelial)
Specification Lung Endothelial
Permeabilization No
Fixative Formaldehyde
Username

Ms. VIctoria Thompson

Verified customer

Submitted Feb 05 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.1M Citrate buffer pH 6.5
Sample Rat Tissue sections (rat pancreas)
Specification rat pancreas
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization Yes - Tween-20 1%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 16 2013



J’ai contacté le laboratoire qui m’a confirmé que tous nos lots d’ab8158 sont testés en immunohistochimie sur coupes paraffines.

Thank you for contacting us.
The isotype controls are as follows:
ab8158 -- ab18450 rat IgG2a
ab51067 -- ab125938 rabbit monoclonal
ab28364 -- ab27472 rabbit polyclonal
I hope this information is helpful to you. Please do not h...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (6-week old testes)
Specification 6-week old testes
Fixative 4% PFA
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Qing Wen

Verified customer

Submitted Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate PH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 18 2012

Thank you for contacting us. Your credit note ID is xxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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