Anti-CD34 antibody [QBEND/10] (FITC) (ab78165)


  • Product nameAnti-CD34 antibody [QBEND/10] (FITC)
    See all CD34 primary antibodies
  • Description
    Mouse monoclonal [QBEND/10] to CD34 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab78165 recognizes CD34, a heavily glycosylated transmembrane protein, gp 105-120 kDa, expressed on immature human haemopoietic precursor cells, capillary endothelial cells and human myeloid cells in the following categories: subsets of CFU-GEMM and BFU-E, all CFU-GM, granulocyte precursors, monocytes, myeloid leukaemias. ab78165 recognizes the class II epitope showing resistance to neuraminidase enzyme cleavage.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus monkey, Rhesus monkey
    Does not react with: Rat, Sheep, Cow, Dog
  • Immunogen

    Human endothelial vesicles

  • Positive control
    • KG-1 cell line


Our Abpromise guarantee covers the use of ab78165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 10µl per test.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPossible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
    • Tissue specificitySelectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
    • Sequence similaritiesBelongs to the CD34 family.
    • Developmental stageOn early hematopoietic progenitor cells.
    • Post-translational
      Highly glycosylated.
      Phosphorylated on serine residues by PKC.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD34 antibody
      • CD34 antigen antibody
      • CD34 molecule antibody
      • CD34_HUMAN antibody
      • Cluster designation 34 antibody
      • Hematopoietic progenitor cell antigen CD34 antibody
      • HPCA1 antibody
      • Mucosialin antibody
      • OTTHUMP00000034733 antibody
      • OTTHUMP00000034734 antibody
      see all

    Anti-CD34 antibody [QBEND/10] (FITC) images

    • Log fluorescence intensity profile of KG-1 cell line stained with ab78165 at 10ul per test.

    References for Anti-CD34 antibody [QBEND/10] (FITC) (ab78165)

    ab78165 has not yet been referenced specifically in any publications.

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    The concentration of ab78165 is around 100 ug/ml however, the exact concentration is not known.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advi...

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    Comme convenu je vous ai fait parvenir un devis pourune unité d'anti-CD34 (FITC) et une unité d'anti-CD166

    Je me permets de vous rappeller que nous offrons jusqu'au 2 m...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (KG-1 cells (human acute myeloid leukemia cell line)
    Specification KG-1 cells (human acute myeloid leukemia cell line
    Preparation Cell harvesting/tissue preparation method: Cells grown in suspension in RPMI-1640 and centrifuged before staining
    Sample buffer: FACS buffer (PBS 0.5% FCS 0.5M EDTA)
    Fixation None
    Permeabilization No
    Gating Strategy All live cells

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    Submitted Mar 01 2010