The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes IHC-P: 1/10 - 1/20.
We suggested an incubation period of 30-60 minutes at room temperature. Proteolytic treatment is required prior to immunostaining of formalin-fixed paraffin-embedded tissue sections.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMediates cellular binding of particles and immune complexes that have activated complement.
Tissue specificityPresent on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells.
Sequence similaritiesBelongs to the receptors of complement activation (RCA) family. Contains 30 Sushi (CCP/SCR) domains.