• Product nameAnti-CD35 antibody [E11]
    See all CD35 primary antibodies
  • Description
    Mouse monoclonal [E11] to CD35
  • SpecificityDoes not block complement receptor 1 (CR1) activity. No cross reaction with CR2.
  • Tested applicationsSuitable for: WB, Flow Cyt, IHC-P, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Human, Baboon, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Tissue, cells or virus corresponding to CD35. human acute monocytic leukaemia cells.
    Database link: P17927



Our Abpromise guarantee covers the use of ab25 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 223 kDa.
Flow Cyt Use at an assay dependent concentration.

Use 10μl of the diluted antibody to label 106 cells or 100μl of whole blood

IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

acetone fixation


  • FunctionMediates cellular binding of particles and immune complexes that have activated complement.
  • Tissue specificityPresent on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells.
  • Sequence similaritiesBelongs to the receptors of complement activation (RCA) family.
    Contains 30 Sushi (CCP/SCR) domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C3 binding protein antibody
    • C3b/C4b receptor antibody
    • C3BR antibody
    • C4BR antibody
    • CD 35 antibody
    • CD35 antibody
    • CD35 antigen antibody
    • complement component (3b/4b) receptor 1 (Knops blood group) antibody
    • complement component (3b/4b) receptor 1 including Knops blood group system antibody
    • Complement component receptor 1 antibody
    • Complement receptor 1 antibody
    • Complement receptor type 1 antibody
    • CR 1 antibody
    • CR1 antibody
    • CR1_HUMAN antibody
    • KN antibody
    • Knops blood group antigen antibody
    see all

Anti-CD35 antibody [E11] images

  • ab25 (1µg/ml) staining CD35 in human spleen.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-CD35 antibody [E11] (ab25)

This product has been referenced in:
  • Park HJ  et al. Using mutagenesis and structural biology to map the binding site for the Plasmodium falciparum merozoite protein PfRh4 on the human immune adherence receptor. J Biol Chem 289:450-63 (2014). ELISA . Read more (PubMed: 24214979) »
  • LaPan P  et al. Optimization of total protein and activity assays for the detection of MMP-12 in induced human sputum. BMC Pulm Med 10:40 (2010). ELISA ; Human . Read more (PubMed: 20678199) »

See all 3 Publications for this product

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Only the BSA seems to be a problem, its concentration should be 0.1%-0.5%. The ideal way is to remove BSA, we have kit for this http://www.abcam.com/BSA-Removal-Kit-ab173231.html. Alternatively you can give it a ...

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Thank you for contacting us. Samples for this kit have to be prepared as follows: tissues or cells can be homogenized in 4 volumes of Assay Buffer and centrifuged (13,000 x g, 10 min) to remove insoluble material. Serum samples can be directly diluted ...

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