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Product name Anti-CD35 antibody [E11], prediluted See all CD35 primary antibodies
Mouse monoclonal [E11] to CD35, prediluted
Specificity Shows no cross-reaction with CD21. Strongly labels the follicular dendritic cells (FDC).
Tested applications Suitable for:
IHC-P more details
Human, Baboon, Rhesus monkey
Intact human monocytes
Epitope Distal from the C3b-binding site.
Unable to block CD35 activity.
Storage instructions Shipped at 4°C. Store at +4°C.
Storage buffer Preservative: 15mM Sodium Azide
Constituents: 0.5M Tris HCl, stabilizing protein, pH 7.6
Concentration information loading...
Purity Protein G purified
Primary antibody notes Unable to block CD35 activity.
Clone number E11
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Pre-diluted ready for use. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 mins, followed by cooling at RT for 20 mins.
Function Mediates cellular binding of particles and immune complexes that have activated complement.
Tissue specificity Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells.
Sequence similarities Belongs to the receptors of complement activation (RCA) family. Contains 30 Sushi (CCP/SCR) domains.
Cellular localization Membrane.
Information by UniProt
C3 binding protein antibody
C3b/C4b receptor antibody
Anti-CD35 antibody [E11], prediluted images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD35 antibody [E11], prediluted (ab74318)
Cell membrane staining of follicular dendritic cells, B cells and granulocytes in formalin-fixed, paraffin-embedded human tonsil tissue sections using ab74318 with a peroxidase-conjugated secondary antibody and a AEC chromogen.
References for Anti-CD35 antibody [E11], prediluted (ab74318)
This product has been referenced in:
et al. Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1). Eur J Immunol 14:236-43 (1984).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"