Overview

  • Product name
    Anti-CD35 antibody [EPR6602]
    See all CD35 primary antibodies
  • Description
    Rabbit monoclonal [EPR6602] to CD35
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acid residues at the C-terminus of Human CD35.

  • Positive control
    • TF1 and Human tonsil lysates; Human kidney and tonsil tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 223 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Mediates cellular binding of particles and immune complexes that have activated complement.
    • Tissue specificity
      Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells.
    • Sequence similarities
      Belongs to the receptors of complement activation (RCA) family.
      Contains 30 Sushi (CCP/SCR) domains.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • C3 binding protein antibody
      • C3b/C4b receptor antibody
      • C3BR antibody
      • C4BR antibody
      • CD 35 antibody
      • CD35 antibody
      • CD35 antigen antibody
      • complement component (3b/4b) receptor 1 (Knops blood group) antibody
      • complement component (3b/4b) receptor 1 including Knops blood group system antibody
      • Complement component receptor 1 antibody
      • Complement receptor 1 antibody
      • Complement receptor type 1 antibody
      • CR 1 antibody
      • CR1 antibody
      • CR1_HUMAN antibody
      • KN antibody
      • Knops blood group antigen antibody
      see all

    Images

    • ab126737 at 1/250 dilution, staining CD35 in Formalin fixed Paraffin-embedded Human tonsil tissue by Immunohistochemistry.
    • ab126737 at 1/250 dilution, staining CD35 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • All lanes : Anti-CD35 antibody [EPR6602] (ab126737) at 1/1000 dilution

      Lane 1 : TF1 lysate
      Lane 2 : Human tonsil lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat-anti-rabbit HRP at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 223 kDa

    References

    This product has been referenced in:
    • Ohe R  et al. Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues. Leuk Lymphoma 57:1687-96 (2016). IHC ; Human . Read more (PubMed: 26700650) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab126737.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up