• Product nameAnti-CD36 antibody [FA6-152]
    See all CD36 primary antibodies
  • Description
    Mouse monoclonal [FA6-152] to CD36
  • Tested applicationsSuitable for: Blocking, Functional Studies, IHC-Fr, Flow Cytmore details
    Unsuitable for: IHC-P or WB
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    20-weeks-old fetal erythrocytes.

  • Positive control
    • HEL cells
  • General notes

    We have data from customer abReviews and publications indicating that this antibody is capable of detecting CD36 via western blot. However, based on customer feedback of difficulties and extensive troubleshooting using this antibody in western blot, we are removing WB as a tested application and recommend ab133625 as an alternative for use in this application.


Our Abpromise guarantee covers the use of ab17044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration. Blocks collagen/thrombospondin binding.
Functional Studies Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IHC-P or WB.
  • Target

    • FunctionMultifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
      (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
    • Involvement in diseasePlatelet glycoprotein IV deficiency
      Coronary heart disease 7
    • Sequence similaritiesBelongs to the CD36 family.
    • Post-translational
      N-glycosylated and O-glycosylated with a ratio of 2:1.
      Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).
    • Cellular localizationCell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adipocyte membrane protein antibody
      • CD36 antibody
      • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
      • CD36 antigen antibody
      • CD36 molecule (thrombospondin receptor) antibody
      • CD36 molecule antibody
      • CD36_HUMAN antibody
      • CHDS7 antibody
      • Cluster determinant 36 antibody
      • Collagen receptor, platelet antibody
      • FAT antibody
      • Fatty acid translocase antibody
      • Fatty acid transport protein antibody
      • Glycoprotein IIIb antibody
      • GP IIIb antibody
      • GP3B antibody
      • GP4 antibody
      • GPIIIB antibody
      • GPIV antibody
      • Leukocyte differentiation antigen CD36 antibody
      • MGC108510 antibody
      • MGC91634 antibody
      • PAS 4 protein antibody
      • PAS IV antibody
      • PAS-4 antibody
      • PASIV antibody
      • Platelet collagen receptor antibody
      • Platelet glycoprotein 4 antibody
      • Platelet glycoprotein IV antibody
      • scarb3 antibody
      • Scavenger receptor class B member 3 antibody
      • Thrombospondin receptor antibody
      see all

    Anti-CD36 antibody [FA6-152] images

    • Overlay histogram showing THP1 cells stained with ab17044 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17044, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed THP1 cells used under the same conditions.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    References for Anti-CD36 antibody [FA6-152] (ab17044)

    This product has been referenced in:
    • Olivetta E  et al. HIV-1 Nef impairs key functional activities in human macrophages through CD36 downregulation. PLoS One 9:e93699 (2014). Flow Cyt ; Human . Read more (PubMed: 24705461) »
    • Wang Y  et al. Platelet-derived S100 family member myeloid-related protein-14 regulates thrombosis. J Clin Invest 124:2160-71 (2014). Mouse . Read more (PubMed: 24691441) »

    See all 16 Publications for this product

    Product Wall

    Thank you for taking the time to send us your results. I am sorry to hear you have had difficulty obtaining satisfactory results from this CD36 antibody as well.

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    Application Blocking
    Sample Mouse Cell (mouse macrophage cell line(raw264.7 cell))
    Specification mouse macrophage cell line(raw264.7 cell)

    Abcam user community

    Verified customer

    Submitted Nov 27 2014

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this our Anti-CD36 antibody [FA6-152] (ab17044) .

    The details you have kindly provided ...

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    Thank you for contacting us.

    The immunogen for ab17044 is 20 weeks old fetal erythrocytes. However, we have not further mapped the epitope for this antibody.

    I hope this information is helpful to you. Please do not hesitate to cont...

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Thank you for filling in the questionnaire. I am sorry to hear that you are still experiencing problems with this antibody.
    I have looked through your protocol and would like to ask you some questions in order to understand your procedure better a...

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    According to our information, we have that platelet aggregation and secretion was induced by > 1μg/ml of this antibody, according to this reference: Kieffer N et al; Development regulated expression of a 78...

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    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
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