Anti-CD36 antibody [MF3] - Low endotoxin, Azide free (ab80080)

Overview

  • Product nameAnti-CD36 antibody [MF3] - Low endotoxin, Azide freeSee all CD36 primary antibodies ...
  • Description
    Rat monoclonal [MF3] to CD36 - Low endotoxin, Azide free
  • SpecificityAntibodies produced by clone MF3 have been shown to inhibit IL4 induced thioglycollate-elicited peritoneal macrophage fusion and significantly block IL4/GM-CSF-induced bone-marrorw derived macrophage fusion.
  • Tested applicationsIHC-P, WB, IP, Functional Studies, Flow Cyt, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    IL4 treated murine thioglycollate-elicited peritoneal macrophages.

  • Positive control
    • Mouse peripheral blood platelets.
  • General notesEndotoxin Level <0.01EU/ug.

Properties

Applications

Our Abpromise guarantee covers the use of ab80080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent dilution. Predicted molecular weight: 53 kDa.
IP Use at an assay dependent dilution.
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use 10µl for 106 cells.
ICC/IF Use at an assay dependent dilution.
ICC Use at an assay dependent concentration.

Target

  • FunctionSeems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic efects.
  • Involvement in diseaseDefects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal.
    Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7) [MIM:610938].
  • Sequence similaritiesBelongs to the CD36 family.
  • Post-translational
    modifications
    N-glycosylated and O-glycosylated with a ratio of 2:1.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adipocyte membrane protein antibody
    • CD 36 antibody
    • CD36 antibody
    • CD36 antibody
    • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
    • CD36 antigen antibody
    • CD36 molecule (thrombospondin receptor) antibody
    • CD36 molecule antibody
    • CD36_HUMAN antibody
    • CHDS7 antibody
    • Cluster determinant 36 antibody
    • Collagen receptor, platelet antibody
    • FAT antibody
    • Fatty acid translocase antibody
    • Fatty acid transport protein antibody
    • Glycoprotein IIIb antibody
    • GP IIIb antibody
    • gp3b antibody
    • gp4 antibody
    • gpiiib antibody
    • gpiv antibody
    • Leukocyte differentiation antigen cd36 antibody
    • MGC108510 antibody
    • MGC91634 antibody
    • PAS 4 antibody
    • PAS 4 protein antibody
    • PAS IV antibody
    • PAS-4 antibody
    • PAS4 antibody
    • pas4 protein antibody
    • pasiv antibody
    • Platelet collagen receptor antibody
    • Platelet glycoprotein 4 antibody
    • Platelet glycoprotein IV antibody
    • scarb3 antibody
    • Scavenger receptor class B member 3 antibody
    • Scavenger receptor class B, member 3 antibody
    • Thrombospondin receptor antibody
    • Thrombospondin receptor cluster determinant 36 antibody
    see all

Anti-CD36 antibody [MF3] - Low endotoxin, Azide free images

  • ab80080 at 10µl/106 cells staining CD36 in mouse peripheral blood platelets by Flow Cytometry.
  • ab80080 staining CD36 in human coronary atherosclerosis plaques by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 0.1% BSA for 30 minutes at 36°C followed by incubation with the primary antibody at a 1/150 dilution for 24 hours at 4°C. A biotin conjugated rabbit polyclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview

  • ab80080 staining CD36 - Low Endotoxin (green) in Mouse RAW 264.7 cells by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized in 0.1% Triton X-100 in 2% BSA for 15 minutes and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA) for 18 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rat IgG polyclonal (1/750) was used as the secondary antibody.
    WGA (red) = Wheat Germ Agglutinin

    See Abreview

References for Anti-CD36 antibody [MF3] - Low endotoxin, Azide free (ab80080)

This product has been referenced in:
  • Xiong W  et al. Induction of TGF-ß1 synthesis by macrophages in response to apoptotic cells requires activation of the scavenger receptor CD36. PLoS One 8:e72772 (2013). WB . Read more (PubMed: 23936544) »
  • Helming L  et al. The scavenger receptor CD36 plays a role in cytokine-induced macrophage fusion. J Cell Sci 122:453-9 (2009). Neutralising ; Mouse . Read more (PubMed: 19155290) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Application Immunocytochemistry
Sample Mouse Cell (RAW 264.7)
Specification RAW 264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted May 23 2012

Thank you for contacting us.

We have a number of antibodies to CD36 that are guaranteed to detect endogenous expression. For example, we have the mouse monoclonal ab17044 which reacts with human and rat for use in blocking, ICC/IF, WB, IHC-P,...

Read More

Thank you for contacting us. The following article describes development of the ab80080 clone, MF3, and how it was used in a neutralization assay. As I mentioned in our conversation, you will want to decide if the neutralization assay has any releva...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (Monocytes from Apheresis)
Loading amount 20 µg
Specification Monocytes from Apheresis
Gel Running Conditions Reduced Denaturing (8-16% Biorad Criterion Gel with 18 wells)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 12 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human coronary atherosclerosis plaques)
Specification human coronary atherosclerosis plaques
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 36°C
Username

Dr. Jaykumar Thumar

Verified customer

Submitted Dec 31 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"