Anti-CD36 antibody [MF3] - Low endotoxin, Azide free (ab80080)

Overview

  • Product nameAnti-CD36 antibody [MF3] - Low endotoxin, Azide free
    See all CD36 primary antibodies
  • Description
    Rat monoclonal [MF3] to CD36 - Low endotoxin, Azide free
  • SpecificityAntibodies produced by clone MF3 have been shown to inhibit IL4 induced thioglycollate-elicited peritoneal macrophage fusion and significantly block IL4/GM-CSF-induced bone-marrorw derived macrophage fusion.
  • Tested applicationsSuitable for: IHC-P, WB, IP, Functional Studies, Flow Cyt, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    IL4 treated murine thioglycollate-elicited peritoneal macrophages.

  • Positive control
    • Mouse peripheral blood platelets.
  • General notesEndotoxin Level <0.01EU/ug.

Applications

Our Abpromise guarantee covers the use of ab80080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • FunctionMultifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
    (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
  • Involvement in diseasePlatelet glycoprotein IV deficiency
    Coronary heart disease 7
  • Sequence similaritiesBelongs to the CD36 family.
  • Post-translational
    modifications
    N-glycosylated and O-glycosylated with a ratio of 2:1.
    Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).
  • Cellular localizationCell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adipocyte membrane protein antibody
    • CD36 antibody
    • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
    • CD36 antigen antibody
    • CD36 molecule (thrombospondin receptor) antibody
    • CD36 molecule antibody
    • CD36_HUMAN antibody
    • CHDS7 antibody
    • Cluster determinant 36 antibody
    • Collagen receptor, platelet antibody
    • FAT antibody
    • Fatty acid translocase antibody
    • Fatty acid transport protein antibody
    • Glycoprotein IIIb antibody
    • GP IIIb antibody
    • GP3B antibody
    • GP4 antibody
    • GPIIIB antibody
    • GPIV antibody
    • Leukocyte differentiation antigen CD36 antibody
    • MGC108510 antibody
    • MGC91634 antibody
    • PAS 4 protein antibody
    • PAS IV antibody
    • PAS-4 antibody
    • PASIV antibody
    • Platelet collagen receptor antibody
    • Platelet glycoprotein 4 antibody
    • Platelet glycoprotein IV antibody
    • scarb3 antibody
    • Scavenger receptor class B member 3 antibody
    • Thrombospondin receptor antibody
    see all

Anti-CD36 antibody [MF3] - Low endotoxin, Azide free images

  • ab80080 at 10µl/106 cells staining CD36 in mouse peripheral blood platelets by Flow Cytometry.
  • ab80080 staining CD36 in human coronary atherosclerosis plaques by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 0.1% BSA for 30 minutes at 36°C followed by incubation with the primary antibody at a 1/150 dilution for 24 hours at 4°C. A biotin conjugated rabbit polyclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview

  • ab80080 staining CD36 - Low Endotoxin (green) in Mouse RAW 264.7 cells by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized in 0.1% Triton X-100 in 2% BSA for 15 minutes and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA) for 18 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rat IgG polyclonal (1/750) was used as the secondary antibody.
    WGA (red) = Wheat Germ Agglutinin

    See Abreview

References for Anti-CD36 antibody [MF3] - Low endotoxin, Azide free (ab80080)

This product has been referenced in:
  • Xiong W  et al. Induction of TGF-ß1 synthesis by macrophages in response to apoptotic cells requires activation of the scavenger receptor CD36. PLoS One 8:e72772 (2013). WB . Read more (PubMed: 23936544) »
  • Helming L  et al. The scavenger receptor CD36 plays a role in cytokine-induced macrophage fusion. J Cell Sci 122:453-9 (2009). Neutralising ; Mouse . Read more (PubMed: 19155290) »

See all 2 Publications for this product

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cell (RAW 264.7)
Specification RAW 264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted May 23 2012

Thank you for contacting us.

We have a number of antibodies to CD36 that are guaranteed to detect endogenous expression. For example, we have the mouse monoclonal ab17044 which reacts with human and rat for use in blocking, ICC/IF, WB, IHC-P,...

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Thank you for contacting us. The following article describes development of the ab80080 clone, MF3, and how it was used in a neutralization assay. As I mentioned in our conversation, you will want to decide if the neutralization assay has any releva...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Monocytes from Apheresis)
Loading amount 20 µg
Specification Monocytes from Apheresis
Gel Running Conditions Reduced Denaturing (8-16% Biorad Criterion Gel with 18 wells)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human coronary atherosclerosis plaques)
Specification human coronary atherosclerosis plaques
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 36°C
Username

Dr. Jaykumar Thumar

Verified customer

Submitted Dec 31 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"