Overview

  • Product nameAnti-CD38 antibody [EPR4106]
    See all CD38 primary antibodies
  • Description
    Rabbit monoclonal [EPR4106] to CD38
  • SpecificityNot suitable for mouse and rat samples because antibody shows a non-specific cytoplasmic staining in almost all tested mouse and rat tissues.
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD38 (C terminal).
    (Peptide available as ab194748)

  • Positive control
    • WB: HeLa, THP-1, Raji and HuT-78 cell lysates, Human plasma and fetal thymus tissue lysates. IHC-P: Human endometrial adenocarcinoma, tonsil, lymph node, skeletal muscle and normal colon tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-CD38 antibody (HRP) [EPR4106] (ab200929)

    Anti-CD38 antibody (Biotin) [EPR4106] (ab201498)

Properties

Applications

Our Abpromise guarantee covers the use of ab108403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).Can be blocked with CD38 peptide (ab194748).

For unpurified use at 1/1000 - 1/10000.

IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/100 - 1/250.

  • Application notesIs unsuitable for Flow Cyt or ICC/IF.
  • Target

    • FunctionSynthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
    • Tissue specificityExpressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
    • Sequence similaritiesBelongs to the ADP-ribosyl cyclase family.
    • Developmental stagePreferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • FormThere are 2 isoforms produced by alternative splicing.
    • Alternative names
      • Acute lymphoblastic leukemia cells antigen CD38 antibody
      • ADP ribosyl cyclase 1 antibody
      • ADP ribosyl cyclase antibody
      • ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
      • ADP-ribosyl cyclase 1 antibody
      • ADPRC 1 antibody
      • cADPr hydrolase 1 antibody
      • CD 38 antibody
      • CD38 antibody
      • CD38 antigen (p45) antibody
      • CD38 antigen antibody
      • CD38 molecule antibody
      • Cd38-rs1 antibody
      • CD38_HUMAN antibody
      • CD38H antibody
      • Cyclic ADP ribose hydrolase antibody
      • Cyclic ADP ribose hydrolase 1 antibody
      • Cyclic ADP-ribose hydrolase 1 antibody
      • EC 3.2.2.5 antibody
      • Ecto nicotinamide adenine dinucleotide glycohydrolase antibody
      • I-19 antibody
      • I19 (mouse) antibody
      • Lymphocyte differentiation antigen CD38 antibody
      • NAD(+) nucleosidase antibody
      • NIM-R5 antigen antibody
      • NIMR5 antigen (mouse) antibody
      • OTTHUMP00000158633 antibody
      • OTTHUMP00000217743 antibody
      • p45 antibody
      • T10 antibody
      see all

    Anti-CD38 antibody [EPR4106] images

    • All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/2000 dilution (purified)

      Lane 1 : HuT-78 cell lysate
      Lane 2 : Human fetal thymus tissue lysate
      Lane 3 : HuT-78 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 34 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : Human plasma cell lysate
      Lane 3 : THP-1 cell lysate
      Lane 4 : HuT-78 cell lysate
      Lane 5 : Human fetal thymus cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 34 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial adenocarcinoma tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling CD38 with unpurified ab108403.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CD38 with unpurified ab108403.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-CD38 antibody [EPR4106] (ab108403)

    This product has been referenced in:
    • Chini CC  et al. Targeting of NAD metabolism in pancreatic cancer cells: potential novel therapy for pancreatic tumors. Clin Cancer Res 20:120-30 (2014). WB ; Human . Read more (PubMed: 24025713) »
    • Kobayashi M  et al. Clinicopathological study of primary biliary cirrhosis with interface hepatitis compared to autoimmune hepatitis. World J Gastroenterol 20:3597-608 (2014). IHC-P ; Human . Read more (PubMed: 24707143) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your enquiry.

    Having contacted the originators of these antibodies, we can let you know the following about the immunogens:

    ab2577: Tissue / cell preparation (Human thymocytes in foetus). According to our information...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"