Overview

  • Product nameAnti-CD38 antibody [EPR4106]
    See all CD38 primary antibodies
  • Description
    Rabbit monoclonal [EPR4106] to CD38
  • SpecificityNot suitable for mouse and rat samples because antibody shows a non-specific cytoplasmic staining in almost all tested mouse and rat tissues.
  • Tested applicationsWB, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues on the C terminus of Human CD38.

  • Positive control
    • WB: HeLa, Human plasma, THP-1, HuT-78 and fetal thymus cell lysates IHC-P: Human endometrial adenocarcinoma and Human tonsil tissues Flow Cyt: HuT-78 cells
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    This product is available conjugated to DyLight® 488 see ab139876.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Applications

Our Abpromise guarantee covers the use of ab108403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).Can be blocked with CD38 peptide (ab194748).
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionSynthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
    • Tissue specificityExpressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
    • Sequence similaritiesBelongs to the ADP-ribosyl cyclase family.
    • Developmental stagePreferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • FormThere are 2 isoforms produced by alternative splicing.
    • Alternative names
      • Acute lymphoblastic leukemia cells antigen CD38 antibody
      • ADP ribosyl cyclase 1 antibody
      • ADP ribosyl cyclase antibody
      • ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
      • ADP-ribosyl cyclase 1 antibody
      • cADPr hydrolase 1 antibody
      • CD 38 antibody
      • CD38 antibody
      • CD38 antigen (p45) antibody
      • CD38 antigen antibody
      • CD38 molecule antibody
      • Cd38-rs1 antibody
      • CD38_HUMAN antibody
      • CD38H antibody
      • Cyclic ADP ribose hydrolase antibody
      • Cyclic ADP ribose hydrolase 1 antibody
      • Cyclic ADP-ribose hydrolase 1 antibody
      • EC 3.2.2.5 antibody
      • Ecto nicotinamide adenine dinucleotide glycohydrolase antibody
      • I-19 antibody
      • I19 (mouse) antibody
      • Lymphocyte differentiation antigen CD38 antibody
      • NAD(+) nucleosidase antibody
      • NIM-R5 antigen antibody
      • NIMR5 antigen (mouse) antibody
      • OTTHUMP00000158633 antibody
      • OTTHUMP00000217743 antibody
      • p45 antibody
      • T10 antibody
      see all

    Anti-CD38 antibody [EPR4106] images

    • All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Human plasma cell lysate
      Lane 3 : THP-1 cell lysate
      Lane 4 : HuT-78 cell lysate
      Lane 5 : fetal thymus cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 34 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    • ab108403, at a dilution of 1/100, staining CD38 in paraffin embedded Human endometrial adenocarcinoma tissue by Immunohistochemistry.
    • ab108403, at a dilution of 1/100, staining CD38 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
    • Flow cytometric analysis of CD38 in HuT-78 cells, using ab108403 (red) at a 1/10 diulution, or a Rabbit IgG (negative) (green).
    • ab108403 showing positive staining in Lymph node tissue.

    • ab108403 showing positive staining in Normal colon tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-CD38 antibody [EPR4106] (ab108403)

    This product has been referenced in:
    • Chini CC  et al. Targeting of NAD metabolism in pancreatic cancer cells: potential novel therapy for pancreatic tumors. Clin Cancer Res 20:120-30 (2014). WB ; Human . Read more (PubMed: 24025713) »
    • Kobayashi M  et al. Clinicopathological study of primary biliary cirrhosis with interface hepatitis compared to autoimmune hepatitis. World J Gastroenterol 20:3597-608 (2014). IHC-P ; Human . Read more (PubMed: 24707143) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your enquiry.

    Having contacted the originators of these antibodies, we can let you know the following about the immunogens:

    ab2577: Tissue / cell preparation (Human thymocytes in foetus). According to our information...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"