Overview

  • Product nameAnti-CD38 antibody [EPR4106]See all CD38 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR4106] to CD38
  • SpecificityNot suitable for mouse and rat samples because antibody shows a non-specific cytoplasmic staining in almost all tested mouse and rat tissues.
  • Tested applicationsWB, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    A synthetic peptide corresponding to residues on the C terminus of Human CD38.

  • Positive control
    • WB: HeLa, Human plasma, THP-1, HuT-78 and fetal thymus cell lysates IHC-P: Human endometrial adenocarcinoma and Human tonsil tissues Flow Cyt: HuT-78 cells
  • General notes

    Produced under U.S. Patent No. 5,675,063.

    This product is available conjugated to DyLight® 488 see ab139876.

Applications

Our Abpromise guarantee covers the use of ab108403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionSynthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
    • Tissue specificityExpressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
    • Sequence similaritiesBelongs to the ADP-ribosyl cyclase family.
    • Developmental stagePreferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • FormThere are 2 isoforms produced by alternative splicing.
    • Alternative names
      • Acute lymphoblastic leukemia cells antigen CD38 antibody
      • ADP ribosyl cyclase 1 antibody
      • ADP ribosyl cyclase antibody
      • ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
      • ADP-ribosyl cyclase 1 antibody
      • cADPr hydrolase 1 antibody
      • CD 38 antibody
      • CD38 antibody
      • CD38 antigen (p45) antibody
      • CD38 antigen antibody
      • CD38 molecule antibody
      • Cd38-rs1 antibody
      • CD38_HUMAN antibody
      • CD38H antibody
      • Cyclic ADP ribose hydrolase antibody
      • Cyclic ADP ribose hydrolase 1 antibody
      • Cyclic ADP-ribose hydrolase 1 antibody
      • EC 3.2.2.5 antibody
      • Ecto nicotinamide adenine dinucleotide glycohydrolase antibody
      • I-19 antibody
      • I19 (mouse) antibody
      • Lymphocyte differentiation antigen CD38 antibody
      • NAD(+) nucleosidase antibody
      • NIM-R5 antigen antibody
      • NIMR5 antigen (mouse) antibody
      • OTTHUMP00000158633 antibody
      • OTTHUMP00000217743 antibody
      • p45 antibody
      • T10 antibody
      see all

    Anti-CD38 antibody [EPR4106] images

    • All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Human plasma cell lysate
      Lane 3 : THP-1 cell lysate
      Lane 4 : HuT-78 cell lysate
      Lane 5 : fetal thymus cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 34 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    • ab108403, at a dilution of 1/100, staining CD38 in paraffin embedded Human endometrial adenocarcinoma tissue by Immunohistochemistry.
    • ab108403, at a dilution of 1/100, staining CD38 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
    • Flow cytometric analysis of CD38 in HuT-78 cells, using ab108403 (red) at a 1/10 diulution, or a Rabbit IgG (negative) (green).
    • ab108403 showing positive staining in Lymph node tissue.

    • ab108403 showing positive staining in Normal colon tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-CD38 antibody [EPR4106] (ab108403)

    This product has been referenced in:
    • Chini CC  et al. Targeting of NAD metabolism in pancreatic cancer cells: potential novel therapy for pancreatic tumors. Clin Cancer Res 20:120-30 (2014). WB ; Human . Read more (PubMed: 24025713) »
    • Kobayashi M  et al. Clinicopathological study of primary biliary cirrhosis with interface hepatitis compared to autoimmune hepatitis. World J Gastroenterol 20:3597-608 (2014). IHC-P ; Human . Read more (PubMed: 24707143) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your enquiry.

    Having contacted the originators of these antibodies, we can let you know the following about the immunogens:

    ab2577: Tissue / cell preparation (Human thymocytes in foetus). According to our information...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"