The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesHIV Syndromes and other clinical situations characterized by lymphocyte in vivoactivation in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
CD38 (FITC) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD38 (FITC) is sufficient for labelling of 1x106 cells.
FunctionSynthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
Tissue specificityExpressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
Sequence similaritiesBelongs to the ADP-ribosyl cyclase family.
Developmental stagePreferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.