• Product nameAnti-CD38 antibody [HIT2]
    See all CD38 primary antibodies
  • Description
    Mouse monoclonal [HIT2] to CD38
  • SpecificityCD38 antigen
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human thymocytes in foetus).

  • Positive control
    • Cell lines derived from B-cell chronic lymphocytic leukemia (B-CLL).



Our Abpromise guarantee covers the use of ab2577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    WB: Use at an assay dependant concentration. Use under non-reducing conditions.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSynthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
    • Tissue specificityExpressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
    • Sequence similaritiesBelongs to the ADP-ribosyl cyclase family.
    • Developmental stagePreferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • FormThere are 2 isoforms produced by alternative splicing.
    • Alternative names
      • Acute lymphoblastic leukemia cells antigen CD38 antibody
      • ADP ribosyl cyclase 1 antibody
      • ADP ribosyl cyclase antibody
      • ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
      • ADP-ribosyl cyclase 1 antibody
      • ADPRC 1 antibody
      • ADPRC1 antibody
      • cADPr hydrolase 1 antibody
      • CD 38 antibody
      • CD38 antibody
      • CD38 antigen (p45) antibody
      • CD38 antigen antibody
      • CD38 molecule antibody
      • Cd38-rs1 antibody
      • CD38_HUMAN antibody
      • CD38H antibody
      • Cyclic ADP ribose hydrolase antibody
      • Cyclic ADP ribose hydrolase 1 antibody
      • Cyclic ADP-ribose hydrolase 1 antibody
      • EC antibody
      • Ecto nicotinamide adenine dinucleotide glycohydrolase antibody
      • I-19 antibody
      • I19 (mouse) antibody
      • Lymphocyte differentiation antigen CD38 antibody
      • NAD(+) nucleosidase antibody
      • NIM-R5 antigen antibody
      • NIMR5 antigen (mouse) antibody
      • OTTHUMP00000158633 antibody
      • OTTHUMP00000217743 antibody
      • p45 antibody
      • T10 antibody
      see all

    Anti-CD38 antibody [HIT2] images

    • Flow cytometry analysis of human peripheral blood leukocytes with anti-human CD38 (HIT2) purified. 

    References for Anti-CD38 antibody [HIT2] (ab2577)

    This product has been referenced in:
    • Joshua D  et al. The labelling index of primitive plasma cells determines the clinical behaviour of patients with myelomatosis. Br J Haematol 94:76-81 (1996). Read more (PubMed: 8757512) »
    • Schlossman (Eds.) et al. Leucocyte typing V. Oxford University Press : (1995). Read more (PubMed: ) »

    See all 4 Publications for this product

    Product Wall

    Thank you for your enquiry.

    Having contacted the originators of these antibodies, we can let you know the following about the immunogens:

    ab2577: Tissue / cell preparation (Human thymocytes in foetus). According to our information...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (b cell)
    Specification b cell
    Permeabilization No
    Gating Strategy LIVE CELLS

    Abcam user community

    Verified customer

    Submitted May 12 2011

    The antibody is routinely tested by flow cytometry not by Western Blotting. The cell lines derived from B-cell chronic lymphocytic leukemia (B-CLL) should be the positive control.

    You should avoid reducing conditions as you propose - the protocol is very general (classical Laemmli buffer). Use SDS gels (SDS is not reducing but denaturing agent) without adding reducing agents (bME, DTT) in the sample buffer.