Overview

  • Product nameAnti-CD38 antibody [HIT2]
    See all CD38 primary antibodies
  • Description
    Mouse monoclonal [HIT2] to CD38
  • SpecificityCD38 antigen
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human thymocytes in foetus).

  • Positive control
    • Cell lines derived from B-cell chronic lymphocytic leukemia (B-CLL).

Properties

Applications

Our Abpromise guarantee covers the use of ab2577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Use under non-reducing conditions.

Target

  • FunctionSynthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
  • Tissue specificityExpressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
  • Sequence similaritiesBelongs to the ADP-ribosyl cyclase family.
  • Developmental stagePreferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing.
  • Alternative names
    • Acute lymphoblastic leukemia cells antigen CD38 antibody
    • ADP ribosyl cyclase 1 antibody
    • ADP ribosyl cyclase antibody
    • ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
    • ADP-ribosyl cyclase 1 antibody
    • ADPRC 1 antibody
    • ADPRC1 antibody
    • cADPr hydrolase 1 antibody
    • CD 38 antibody
    • CD38 antibody
    • CD38 antigen (p45) antibody
    • CD38 antigen antibody
    • CD38 molecule antibody
    • Cd38-rs1 antibody
    • CD38_HUMAN antibody
    • CD38H antibody
    • Cyclic ADP ribose hydrolase antibody
    • Cyclic ADP ribose hydrolase 1 antibody
    • Cyclic ADP-ribose hydrolase 1 antibody
    • EC 3.2.2.5 antibody
    • Ecto nicotinamide adenine dinucleotide glycohydrolase antibody
    • I-19 antibody
    • I19 (mouse) antibody
    • Lymphocyte differentiation antigen CD38 antibody
    • NAD(+) nucleosidase antibody
    • NIM-R5 antigen antibody
    • NIMR5 antigen (mouse) antibody
    • OTTHUMP00000158633 antibody
    • OTTHUMP00000217743 antibody
    • p45 antibody
    • T10 antibody
    see all

Anti-CD38 antibody [HIT2] images

  • Flow cytometry analysis of human peripheral blood leukocytes with anti-human CD38 (HIT2) purified. 

References for Anti-CD38 antibody [HIT2] (ab2577)

This product has been referenced in:
  • Park JS  et al. Therapeutic effects of mouse bone marrow-derived clonal mesenchymal stem cells in a mouse model of inflammatory bowel disease. J Clin Biochem Nutr 57:192-203 (2015). IHC-Fr ; Mouse . Read more (PubMed: 26566304) »
  • Joshua D  et al. The labelling index of primitive plasma cells determines the clinical behaviour of patients with myelomatosis. Br J Haematol 94:76-81 (1996). Read more (PubMed: 8757512) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry.

Having contacted the originators of these antibodies, we can let you know the following about the immunogens:

ab2577: Tissue / cell preparation (Human thymocytes in foetus). According to our information...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (b cell)
Specification b cell
Permeabilization No
Gating Strategy LIVE CELLS
Username

Abcam user community

Verified customer

Submitted May 12 2011

The antibody is routinely tested by flow cytometry not by Western Blotting. The cell lines derived from B-cell chronic lymphocytic leukemia (B-CLL) should be the positive control.

You should avoid reducing conditions as you propose - the protocol is very general (classical Laemmli buffer). Use SDS gels (SDS is not reducing but denaturing agent) without adding reducing agents (bME, DTT) in the sample buffer.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"