• Product nameAnti-CD3D antibody [EP4426]
    See all CD3D primary antibodies
  • Description
    Rabbit monoclonal [EP4426] to CD3D
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD3D (C terminal).

  • Positive control
    • Jurkat, HuT 78 and Human thymus lysates; Human tonsil tissue.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.



Our Abpromise guarantee covers the use of ab109531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • FunctionThe CD3 complex mediates signal transduction.
  • Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • Sequence similaritiesContains 1 ITAM domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Form.
  • Alternative names
    • CD3 antigen delta subunit antibody
    • CD3 delta antibody
    • CD3d antibody
    • CD3d antigen delta polypeptide (TiT3 complex) antibody
    • CD3d molecule delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • IMD19 antibody
    • OKT3 delta chain antibody
    • T cell receptor T3 delta chain antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T3D antibody
    see all

Anti-CD3D antibody [EP4426] images

  • ab109531, at 1/100 dilution, staining paraffin embedded Human tonsil tissue by Immunohistochemistry.
  • ab109531 staining CD3 in Jurkat cells by Flow Cytometry. Cells were fixed in paraformaldehyde and permeabilized in saponin. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at 4°C. A phycoerythrin-conjugated Goat anti-rabbit IgG (1/100) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-CD3D antibody [EP4426] (ab109531) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HuT 78 cell lysate
    Lane 3 : Human thymus tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size : 19 kDa

References for Anti-CD3D antibody [EP4426] (ab109531)

This product has been referenced in:
  • Alunno A  et al. Telocytes in minor salivary glands of primary Sjögren's syndrome: association with the extent of inflammation and ectopic lymphoid neogenesis. J Cell Mol Med 19:1689-96 (2015). IHC-P ; Human . Read more (PubMed: 25753463) »

See 1 Publication for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Sample Human Cell (Jurkat (T lymphocytes))
Specification Jurkat (T lymphocytes)
Permeabilization Yes - SAPONIN

Ms. Beatriz Garcillan

Verified customer

Submitted Oct 15 2013

Vielen dank fuer Ihren Anruf. Ich habe nun ein paar Antikoerper fuer Sie herrausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und fuer IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links ...

Read More