• Product nameAnti-CD3D antibody [EP4426]
    See all CD3D primary antibodies
  • Description
    Rabbit monoclonal [EP4426] to CD3D
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD3D (C terminal).

  • Positive control
    • Jurkat, HuT 78 and Human thymus lysates; Human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-CD3 antibody (Alexa Fluor® 488) [EP4426] (ab198935)

    Anti-CD3 antibody (Alexa Fluor® 647) [EP4426] (ab198937)

    Anti-CD3 antibody (HRP) [EP4426] (ab198936)



Our Abpromise guarantee covers the use of ab109531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • FunctionThe CD3 complex mediates signal transduction.
  • Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • Sequence similaritiesContains 1 ITAM domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Form.
  • Alternative names
    • CD3d antibody
    • CD3D_HUMAN antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody

Anti-CD3D antibody [EP4426] images

  • ab109531, at 1/100 dilution, staining paraffin embedded Human tonsil tissue by Immunohistochemistry.
  • ab109531 staining CD3 in Jurkat cells by Flow Cytometry. Cells were fixed in paraformaldehyde and permeabilized in saponin. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at 4°C. A phycoerythrin-conjugated Goat anti-rabbit IgG (1/100) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-CD3D antibody [EP4426] (ab109531) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HuT 78 cell lysate
    Lane 3 : Human thymus tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size : 19 kDa

References for Anti-CD3D antibody [EP4426] (ab109531)

This product has been referenced in:
  • Alunno A  et al. Telocytes in minor salivary glands of primary Sjögren's syndrome: association with the extent of inflammation and ectopic lymphoid neogenesis. J Cell Mol Med 19:1689-96 (2015). IHC-P ; Human . Read more (PubMed: 25753463) »

See 1 Publication for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Sample Human Cell (Jurkat (T lymphocytes))
Specification Jurkat (T lymphocytes)
Permeabilization Yes - SAPONIN

Ms. Beatriz Garcillan

Verified customer

Submitted Oct 15 2013

Vielen dank fuer Ihren Anruf. Ich habe nun ein paar Antikoerper fuer Sie herrausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und fuer IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links ...

Read More