Anti-CD4 antibody [74-12-4] (Phycoerythrin) (ab25354)


  • Product nameAnti-CD4 antibody [74-12-4] (Phycoerythrin)
    See all CD4 primary antibodies
  • Description
    Mouse monoclonal [74-12-4] to CD4 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab25354 recognises porcine CD4a.
  • Tested applicationsSuitable for: Flow Cyt, IP, Microcytotoxicity testingmore details
  • Species reactivity
    Reacts with: Pig
  • Immunogen

    The details of the immunogen for this antibody are not available.



Our Abpromise guarantee covers the use of ab25354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Microcytotoxicity testing
  • Application notesFlow Cyt: Use 0.2µg for 106 cells.
    IP: Use at an assay dependent dilution.
    Mct: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt
    • Alternative names
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    Anti-CD4 antibody [74-12-4] (Phycoerythrin) images

    • Flow Cytometry analysis of Porcine peripheral blood lymphocytes labeling CD4 with ab25354 at 0.1 μg/106 cells. A Mouse Anti-Porcine CD8α-FITC was used as the secondary antibody.

    • Peripheral blood mononuclear cells were isolated from heparinized pig blood on Ficoll-Hypaque density gradients and double-stained with ab25354 at 0.2ug/106 cells and mouse anti-pig CD8a-FITC. Lymphocytes were gated andanalyzed on a FACScan• flow cytometer (BDIS, San Jose, CA).

    References for Anti-CD4 antibody [74-12-4] (Phycoerythrin) (ab25354)

    ab25354 has not yet been referenced specifically in any publications.

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