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Post-translational modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
All lanes : Anti-CD4 antibody (ab117306) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Thymus (Human) Tissue Lysate - adult normal tissue Lane 2 : Lymph node (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 25 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 51 kDa Observed band size : 51 kDa
Exposure time : 20 minutes
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab117306 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
References for Anti-CD4 antibody (ab117306)
has not yet been referenced specifically in any publications.
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