• Product nameAnti-CD4 antibody
    See all CD4 primary antibodies
  • Description
    Rabbit polyclonal to CD4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human CD4 aa 200-300 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P01730

  • Positive control
    • This antibody gave a positive signal in Human Thymus and Lymph Node tissue lysates.



Our Abpromise guarantee covers the use of ab117306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).


  • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 antibody images

  • All lanes : Anti-CD4 antibody (ab117306) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : Thymus (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Lymph node (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 25 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 51 kDa

    Exposure time : 20 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab117306 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References for Anti-CD4 antibody (ab117306)

ab117306 has not yet been referenced specifically in any publications.

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