Overview

  • Product nameAnti-CD4 antibody [BC/1F6]
    See all CD4 primary antibodies
  • Description
    Mouse monoclonal [BC/1F6] to CD4
  • Tested applicationsICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human, Monkey, Baboon
  • Immunogen

    Recombinant fragment (Human) corresponding to the external domain

  • Positive control
    • Tonsil or T-cell lymphoma
  • General notesThis antibody works well when used in combination with ab972 - Antigen retrieval solution - heat mediated high pH 9.5.

Properties

Applications

Our Abpromise guarantee covers the use of ab846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The Tris based buffer pH 9.5 is recommended. This antibody may be diluted to a titer of 1/25 - 1/50 in an ABC method. We suggest an incubation period of 1-2 hours at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user. Use peroxidase block after the primary antibody or staining will be diminished!
IHC-Fr Use at an assay dependent dilution. Use peroxidase block after the primary antibody or staining will be diminished. Post-fixation with 10% formalin after the primary antibody may improve antibody staining.

Target

  • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 Antigen antibody
    • CD4 molecule antibody
    • CD4 Receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T Cell Antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T Cell Surface Antigen T4/Leu3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T Cell Surface Glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 antibody [BC/1F6] images

  • ab846 at 1/250 staining human PBMC tissue sections by IHC-P. The tissue was paraformaldehyde fixed and then an antigen retieval step was performed (heat mediated) prior to incubating the cells with the antibody for 20 minutes. A biotinylated goat anti-mouse IgG antibody was used as the secondary.

    See Abreview

  • ab846 staining CD4 in Human inflammatory dermatosis tissue sections by IHC-P (formaldehyde fixed paraffin embedded sections). Tissue was fixed with formaldehyde and blocked by 2% BSA for 30 minutes at 22°C. Antigen retrieval was by heat mediation in citrate buffer (pH 6.0). Samples were incubated with primary antibody (1/25 in BSA/TBS) for 14 hours at 4°C. Ab 6785 (1/800) was used as the secondary antibody

    See Abreview

  • ab846 staining CD4 in human lymphocytes by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in methanol, blocked using 4% BSA for 30 minutes at 25°C and then incubated with ab846 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/100 dilution.

    Double stained with Anti-CD3 antibody [PS1] (ab699), secondary Texas Red.

    See Abreview

  • ab846 staining beta Endorphin in monkey brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and blocked with 5% BSA + 10% goat serum for 1.5 hours at room temp; antigen retrieval was by heat mediation in antigen retrieval buffer. Samples were incubated with primary antibody (1/50 in 5% BSA + 10% goat serum) for 17 hours at 4°C. A biotin-conjugated goat anti-mouse polyclonal IgG (1/300) was used as the secondary antibody. Staining was detected with DAB.

    See Abreview

References for Anti-CD4 antibody [BC/1F6] (ab846)

This product has been referenced in:
  • Kim EJ  et al. Costimulation blockade alters germinal center responses and prevents antibody-mediated rejection. Am J Transplant 14:59-69 (2014). Histology, IF ; Human . Read more (PubMed: 24354871) »
  • Bohne F  et al. HCV-Induced Immune Responses Influence the Development of Operational Tolerance After Liver Transplantation in Humans. Sci Transl Med 6:242ra81 (2014). Human . Read more (PubMed: 24964989) »

See all 7 Publications for this product

Product Wall

Thank you very much for your feedback. It has now been published in the Q/A section.

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Protein Block serum free from Dako as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris buffer pH9.5
Sample Rabbit Tissue sections (Ear wound exudates)
Specification Ear wound exudates
Permeabilization No
Fixative Formaldehyde
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Abcam user community

Verified customer

Submitted Oct 07 2013


For ab846, it is stated on the datasheet that antigen retrieval should be done at a high pH so pH9 would be suitable.

For ab4055 it is not specified what pH should be used so I would recommend heat mediated antigen retrieval either using...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: ph 9,0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (brain tissue of Japanese macaque)
Specification brain tissue of Japanese macaque
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer , pressure cooked for 24 minutes
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 11 2012

Thank you for contacting us.

For ab846, this reference notes the use of this antibody in Rhesus monkey.

http://www.ncbi.nlm.nih.gov/pubmed/22387550?dopt=Abstract

For ab4055, we have not tested Monkey in-house, but this cu...

Read More

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, this antibody has only been tried with an antigen retrieval step in IHC.The Abreviews submitted by other customers for this applications indicate they have all us...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Lymphocytes)
Specification Lymphocytes
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 03 2012

Thank you for your enquiry and your interest. I have checked the datasheets of these three antibodies and though they have been tested for IHC-P, their cross-reactivity with bovine has not been determined yet. I have also conducted a search for...

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