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Recombinant fragment (Human) corresponding to the external domain
Our Abpromise guarantee covers the use of ab846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The Tris based buffer pH 9.5 is recommended. This antibody may be diluted to a titer of 1/25 - 1/50 in an ABC method. We suggest an incubation period of 1-2 hours at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user. Use peroxidase block after the primary antibody or staining will be diminished!|
|IHC-Fr||Use at an assay dependent dilution. Use peroxidase block after the primary antibody or staining will be diminished. Post-fixation with 10% formalin after the primary antibody may improve antibody staining.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"