RabMAb

Anti-CD4 antibody [EPR3155(2)] (ab108329)

Overview

  • Product nameAnti-CD4 antibody [EPR3155(2)]
    See all CD4 primary antibodies
  • Description
    Rabbit monoclonal [EPR3155(2)] to CD4
  • Tested applicationsWB, IP, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to internal sequence amino acids 335-365 of Human CD4

  • Positive control
    • Human fetal thymus, Human tonsil, and PBMC lysates
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab108329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IP 1/10 - 1/100.
ICC 1/500 - 1/1000.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    Anti-CD4 antibody [EPR3155(2)] images

    • All lanes : Anti-CD4 antibody [EPR3155(2)] (ab108329) at 1/1000 dilution

      Lane 1 : Human fetal thymus lysate
      Lane 2 : Human tonsil lysate
      Lane 3 : PBMC lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 51 kDa

    References for Anti-CD4 antibody [EPR3155(2)] (ab108329)

    ab108329 has not yet been referenced specifically in any publications.

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