Overview

  • Product nameAnti-CD4 antibody [GK1.5]See all CD4 primary antibodies ...
  • Description
    Rat monoclonal [GK1.5] to CD4
  • Specificityab25475 recognises CD4/L3T4, Mr 52 kDa.
  • Tested applicationsFunctional Studies, Flow Cyt, IP, IHC-Fr more details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse) - cloned mouse CTL line V4.

  • General notes


    This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

Properties

Applications

Our Abpromise guarantee covers the use of ab25475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent dilution. PubMed: 6415170(In vivo and in vitro depletion of CD4+ cells; Inhibition of T helper function and Fractionation of CD4+ cells) Use at an assay dependent dilution. For details see: Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1. See also PubMed: 6415170. For details see: Kruisbeek, A. M., et al.
Flow Cyt Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent concentration. PubMed: 21345609

Target

  • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Target information above from: UniProt accession P01730 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 Antigen antibody
    • CD4 molecule antibody
    • CD4 Receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T Cell Antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T Cell Surface Antigen T4/Leu3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T Cell Surface Glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

References for Anti-CD4 antibody [GK1.5] (ab25475)

This product has been referenced in:
  • Meshii N  et al. Enhancement of systemic tumor immunity for squamous cell carcinoma cells by an oncolytic herpes simplex virus. Cancer Gene Ther 20:493-8 (2013). IHC-Fr ; Mouse . Read more (PubMed: 23887644) »
  • Billiard F  et al. Abdominal ?-radiation induces an accumulation of function-impaired regulatory T cells in the small intestine. Int J Radiat Oncol Biol Phys 80:869-76 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21345609) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (Liver, Spleen, Lung)
Specification Liver, Spleen, Lung
Permeabilization No
Fixative no fixation
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Submitted Aug 15 2013

I can confirm that these two antibodies ab25478 and ab25475 are tested externally in functional studies.

There are publications where these clones have been tested in this application. I have attached thse for your review and hope this will ...

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Our products of animal origin are derived from animals that have not given evidence of any disease, mentioned in list A of the Office of International des Epizooties (OIE). With that being said, our products are no...

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I have requested further information regarding this from lab. I will be able to send you the details soon.

Many thanks for having patience!

I am very pleased to hear you would like to accept our offer and test ab25475 inIHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forIHC-P and include this code in the &ld...

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I am very pleased to hear you would like to accept our offer and test ab25475 in IHC-P. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forIHC-P and include this code in the...

Read More
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Spleen)
Specification Spleen
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 23 2009

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spleen)
Specification spleen
Fixative Formaldehyde
Permeabilization Yes - 0.05%TritonX-100/PBS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 25°C
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Submitted Dec 12 2008

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (tumor mouse)
Specification tumor mouse
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Miss. Silke Vorwald

Verified customer

Submitted Sep 26 2008

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse skeletal muscle)
Specification mouse skeletal muscle
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Submitted Feb 13 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"