Anti-CD4 antibody [GK1.5] (Allophycocyanin) (ab25497)

Overview

  • Product nameAnti-CD4 antibody [GK1.5] (Allophycocyanin)
    See all CD4 primary antibodies
  • Description
    Rat monoclonal [GK1.5] to CD4 (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Specificityab25497 recognises CD4/L3T4.
  • Tested applicationsIHC-Fr, IP, Functional Studies, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse) - cloned mouse CTL line V4.

  • General notes


    This antibody blocks helper T cell responses to MHC class II antigens,including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

Properties

Applications

Our Abpromise guarantee covers the use of ab25497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
IP
Functional Studies
Flow Cyt
  • Application notesFlow Cyt: Use 0.2µg for 106 cells.
    FuncS (In vivo and in vitro depletion of CD4+ cells; Inhibition of T helper function; Fractionation of CD4+ cells): Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 Antigen antibody
      • CD4 molecule antibody
      • CD4 Receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T Cell Antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T Cell Surface Antigen T4/Leu3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T Cell Surface Glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    References for Anti-CD4 antibody [GK1.5] (Allophycocyanin) (ab25497)

    ab25497 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"