Anti-CD4 antibody [GK1.5] (Biotin) (ab19641)

Overview

  • Product nameAnti-CD4 antibody [GK1.5] (Biotin)
    See all CD4 primary antibodies
  • Description
    Rat monoclonal [GK1.5] to CD4 (Biotin)
  • ConjugationBiotin
  • SpecificityRecognizes a determinant designated L3T4 present on mouse T-cell surface with an apparent molecular mass of 52kDa. The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
  • Tested applicationsFlow Cyt, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Lyt-2-negative T-cell clone 243/2.5

Properties

Applications

Our Abpromise guarantee covers the use of ab19641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
ICC/IF
IP
  • Application notesFlow Cyt: Use 1µg for 106 cells.
    IF: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    Depletion
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 Antigen antibody
      • CD4 molecule antibody
      • CD4 Receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T Cell Antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T Cell Surface Antigen T4/Leu3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T Cell Surface Glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    References for Anti-CD4 antibody [GK1.5] (Biotin) (ab19641)

    ab19641 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample Mouse Tissue sections (Mouse oral tumor cells (MOC-2 obtained from Uppalu)
    Specification Mouse oral tumor cells (MOC-2 obtained from Uppalu
    Permeabilization No
    Fixative Acetone
    Username

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    Verified customer

    Submitted Mar 24 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"