Overview

  • Product nameAnti-CD4 antibody [GK1.5] (Cy5.5 ®)See all CD4 primary antibodies ...
  • Description
    Rat monoclonal [GK1.5] to CD4 (Cy5.5 ®)
  • ConjugationCy5.5 ®. Ex: 678nm, Em: 703nm
  • SpecificityCD4/L3T4, 52 kDa
  • Tested applicationsIHC-Fr, Flow Cyt, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Cloned mouse CTL line V4

  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.


    In vivo and in vitro treatment with the GK1.5 mAb effectively depletes CD4+ cells. The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

Properties

Applications

Our Abpromise guarantee covers the use of ab41467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
Functional Studies Use at an assay dependent dilution.

Target

  • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 Antigen antibody
    • CD4 molecule antibody
    • CD4 Receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T Cell Antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T Cell Surface Antigen T4/Leu3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T Cell Surface Glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

References for Anti-CD4 antibody [GK1.5] (Cy5.5 ®) (ab41467)

This product has been referenced in:
  • Bluestone JA  et al. Repertoire development and ligand specificity of murine TCR gamma delta cells. Immunol Rev 120:5-33 (1991). Read more (PubMed: 1650760) »
  • Havran WL & Fitch FW Effects of anti-Lyt-2 and anti-L3T4 monoclonal antibodies on the function of cytotoxic T lymphocyte/helper T lymphocyte hybrid T cell clones. J Immunol 141:1808-12 (1988). Read more (PubMed: 2459190) »

See all 8 Publications for this product

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Thank you very much for your interest in Abcam and our products..

To our knowledge,the following antibodies you listed have not been tested in IHC-P. Tested applications and species are listed on the individual datasheets. These are updated ...

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Thank you for your enquiry and your patience. Unfortunately, I've confirmed that this product has not been tested specifically for IHC-P, and only for IHC-Fr. I have updated the datasheet to include this information and I'm sorry for any inconvenien...

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