Tissue/ cell preparation (Mouse) - cloned mouse CTL line V4.
This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines. This antibody has been shown to be useful in studies of in vitro and in vivo depletion of CD4+ cells, inhibition of T helper function and fractionation of CD4+ cells.
Our Abpromise guarantee covers the use of ab25342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells. ab154456-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
ab25342 staining CD4 in mouse spleen cells by Flow Cytometry. The sample was incubated with the primary antibody (1/500 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C. Gating Strategy: total splenocytes.
ab25342 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"