Anti-CD4 antibody [GK1.5] (Phycoerythrin) (ab86859)

Overview

  • Product nameAnti-CD4 antibody [GK1.5] (Phycoerythrin)See all CD4 primary antibodies ...
  • Description
    Rat monoclonal [GK1.5] to CD4 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Mouse splenocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab86859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 0.05-0.2 µg for 106 cells.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    Anti-CD4 antibody [GK1.5] (Phycoerythrin) images

    • Flow analysis of CD4 in mouse splenocytes, using 0.05 ug of ab86859 for 106 cells.
      Shaded histogram represents cells without antibody; green represents isotype control; red represents anti CD4 antibody ab86859.
    • Overlay histogram showing Mouse thymocyte cells stained with ab86859 (blue line). The cells dissociated from Thymus tissue into a single cell suspension. Cells were gated on lymphocytes. They were incubated the primary antibody (ab86859, 1µg/1x106 cells) for 30 min. Isotype control antibody (red histogram) was a FITC-conjugated CD8 antibody used under the same conditions.

      See Abreview

    References for Anti-CD4 antibody [GK1.5] (Phycoerythrin) (ab86859)

    ab86859 has not yet been referenced specifically in any publications.

    Product Wall

    Application Flow Cytometry
    Sample Mouse Cell (Thymocytes)
    Specification Thymocytes
    Preparation Cell harvesting/tissue preparation method: Thymus was dissociated into single cell suspension
    Sample buffer: PBS
    Fixation none
    Permeabilization No
    Gating Strategy Lymphocytes
    Username

    Abcam user community

    Verified customer

    Submitted Mar 31 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"