Anti-CD4 antibody [GK1.5] (Phycoerythrin) (ab86859)


  • Product nameAnti-CD4 antibody [GK1.5] (Phycoerythrin)
    See all CD4 primary antibodies
  • Description
    Rat monoclonal [GK1.5] to CD4 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Mouse splenocytes
  • General notesAb136585-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.



Our Abpromise guarantee covers the use of ab86859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 0.05-0.2 µg for 106 cells.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    Anti-CD4 antibody [GK1.5] (Phycoerythrin) images

    • Flow analysis of CD4 in mouse splenocytes, using 0.05 ug of ab86859 for 106 cells.
      Shaded histogram represents cells without antibody; green represents isotype control; red represents anti CD4 antibody ab86859.
    • Overlay histogram showing Mouse thymocyte cells stained with ab86859 (blue line). The cells dissociated from Thymus tissue into a single cell suspension. Cells were gated on lymphocytes. They were incubated the primary antibody (ab86859, 1µg/1x106 cells) for 30 min. Isotype control antibody (red histogram) was a FITC-conjugated CD8 antibody used under the same conditions.

      See Abreview

    References for Anti-CD4 antibody [GK1.5] (Phycoerythrin) (ab86859)

    ab86859 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Mouse Cell (Thymocytes)
    Specification Thymocytes
    Preparation Cell harvesting/tissue preparation method: Thymus was dissociated into single cell suspension
    Sample buffer: PBS
    Fixation none
    Permeabilization No
    Gating Strategy Lymphocytes

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    Submitted Mar 31 2011