Post-translational modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Flow analysis of CD4 in mouse splenocytes, using 0.05 ug of ab86859 for 106 cells. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti CD4 antibody ab86859.
Flow Cytometry - CD4 antibody [GK1.5] (Phycoerythrin) (ab86859)This image is courtesy of an Anonymous Abreview.
Overlay histogram showing Mouse thymocyte cells stained with ab86859 (blue line). The cells dissociated from Thymus tissue into a single cell suspension. Cells were gated on lymphocytes. They were incubated the primary antibody (ab86859, 1µg/1x106 cells) for 30 min. Isotype control antibody (red histogram) was a FITC-conjugated CD8 antibody used under the same conditions.