![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [mAb51312] (ab51312) image](#ab51312_1 copy.jpg)
![Western blot - CD4 antibody [mAb51312] (ab51312) image](#CD4-Primary-antibodies-ab51312-3.jpg)
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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-CD4 antibody [mAb51312]
See all CD4 products (97) ...
Description
Mouse monoclonal [mAb51312] to CD4
Tested applications
IHC-P, WB, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human CD4.
(Peptide available as ab54699.)
Positive control
This antibody gave a positive signal in Human Thymus and Human Lymph Node Tissue Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
mAb51312
Isotype
IgG1
Light chain type
kappa
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
IHC-Fr: Use at an assay dependent concentration. (PubMed: 21690251)
Target
Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modifications
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Target information above from: UniProt accessionP01730
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Database links
- Entrez Gene: 920 Human
- Entrez Gene: 12504 Mouse
- Omim: 186940 Human
- SwissProt: P01730 Human
- SwissProt: P06332 Mouse
- Unigene: 631659 Human
- Unigene: 2209 Mouse
Anti-CD4 antibody [mAb51312] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [mAb51312] (ab51312)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD4 antibody [mAb51312] (ab51312)](/ps/datasheet/Images/51/ab51312/ab51312_1 copy.jpg)
ab51312 staining CD4 in mouse brain tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxidase block and 10 minutes for protein block). The primary antibody was diluted 1/250 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to mouse IgG was used undiluted as secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
Western blot - CD4 antibody [mAb51312] (ab51312)
![Western blot - CD4 antibody [mAb51312] (ab51312)](/ps/datasheet/images/51/ab51312/CD4-Primary-antibodies-ab51312-3.jpg)
All lanes : Anti-CD4 antibody [mAb51312] (ab51312) at 5 µg/ml
Lane 1 : Thymus (Mouse) Tissue Lysate
Lane 2 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)
Lane 3 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 51 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
References for Anti-CD4 antibody [mAb51312] (ab51312)
This product has been referenced in:
- Cheon ECet al. Mast cell 5-lipoxygenase activity promotes intestinal polyposis in APCDelta468 mice. Cancer Res 71:1627-36 (2011). IHC; Mouse.Read more (PubMed: 21216893) »
- Leib Cet al. Role of the cholinergic antiinflammatory pathway in murine autoimmune myocarditis. Circ Res 109:130-40 (2011). Mouse.Read more (PubMed: 21597011) »
See all 3 publications for this product
Publishing research using ab51312? Please let us know so that we can cite the reference in this datasheet
![CD4 antibody [mAb51312] for IHC-P in Mouse (51312)](/ps/Reviews/Images/ab12479_69547.jpg)
![CD4 antibody [mAb51312] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (51312)](/ps/datasheet/images/51/ab51312/CD4-Primary-antibodies-ab51312-4.jpg)
![CD4 antibody [mAb51312] for Immunocytochemistry/ Immunofluorescence in Human (51312)](/ps/datasheet/images/51/ab51312/CD4-Primary-antibodies-ab51312-10.jpg)
![CD4 antibody [mAb51312] for IHC-P in Rat (51312)](/ps/Reviews/Images/ab12480_69551.jpg)
![CD4 antibody [mAb51312] for IHC-P in Human (51312)](/ps/Reviews/Images/ab12478_69596.jpg)
![CD4 antibody [mAb51312] for Flow Cyt in Human (51312)](/ps/Reviews/Images/ab12804_73159.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"