Overview

  • Product nameAnti-CD4 antibody [mAb51312]
    See all CD4 primary antibodies
  • Description
    Mouse monoclonal [mAb51312] to CD4
  • SpecificityNote about mouse cross reactivity: We have had varying results with this antibody in mouse with some batches performing better than others. The immunogen sequence does not predict cross reactivity with mouse, but we and others have previously seen positive results. Due to this variability, we are no longer able to guarantee that ab51312 will cross react in mouse. Please contact Scientific Support if you require any further information.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human CD4.

    (Peptide available as ab54699.)

  • Positive control
    • This antibody gave a positive signal in Western Blot in Human Thymus and Human Lymph Node Tissue Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab51312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).Can be blocked with Human CD4 peptide (ab54699).

Target

  • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 Antigen antibody
    • CD4 molecule antibody
    • CD4 Receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T Cell Antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T Cell Surface Antigen T4/Leu3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T Cell Surface Glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 antibody [mAb51312] images

  • Lanes 1 - 2 : Anti-CD4 antibody [mAb51312] (ab51312) at 5 µg/ml
    Lanes 3 - 4 : Anti-CD4 antibody [mAb51312] (ab51312) at 1 µg/ml

    Lane 1 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)
    Lane 2 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)
    Lane 3 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)
    Lane 4 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-CD4 antibody [mAb51312] (ab51312)

This product has been referenced in:
  • Xiao H  et al. Adenosine A2A receptor: a target for regulating renal interstitial fibrosis in obstructive nephropathy. PLoS One 8:e60173 (2013). IHC-P ; Mouse . Read more (PubMed: 23585831) »
  • Deckert V  et al. Development of abdominal aortic aneurysm is decreased in mice with plasma phospholipid transfer protein deficiency. Am J Pathol 183:975-86 (2013). Read more (PubMed: 23830874) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6
Sample Mouse Tissue sections (Mouse Prostate)
Specification Mouse Prostate
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 20 2013

Thank you for contacting us.

We can suggest the following antibodies;

http://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg1-heavy-chain-fitc-ab97239.html

http://www.abcam.com/goat-polyclonal-secondary-antibody...

Read More

Merci de nous avoir contactés et pour les informations qui vont nous permettre de chercher l'origine du problème rencontré avec le ab846.

Comme convenu j'ai mis en place l'envoi d'une unité de ab51312, le numé...

Read More

Thank you very much for your call today.

Sinceab4055 has not been tested with mouse samples, I can offer a discount off a future purchase if you buy ab4055 now, test it with mouse samples and submit feedback to us in the form of an Abreview. ...

Read More

Thank you for your email.

There may be some antibodies where I might have suggested the human tissues or cells. I apologize for this. I have included the positive control information irrespective of species so similar mouse tissues sections ca...

Read More

Thank you for contacting us.

The positive control for ab36392 is IL2 activatedcultured NK cells.

ab9918; human tonsil sections, human cervical squamous cell carcinoma section

ab34674; human artery or colon tissue sections
Read More

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization Yes - .01% Tween
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 09 2012

Merci beaucoup pour votre appel.

Je suis désolée pour les inconvenances que vous avez eu avec le ab51312. Veuillez s'il vous plaît me confirmer l'adresse de livraison à laquelle vous souhaitez recevoir l'anticorps d...

Read More
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 19 2012

Thank you for contacting us and your help in this matter.

I have added the two free of charge replacements to your next order, which is on picklist 1084891.

I wish your customer best of luck with their research.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"