Biotin Anti-CD4 antibody [MEM-241] (ab28069)
Key features and details
- Biotin Mouse monoclonal [MEM-241] to CD4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-CD4 antibody [MEM-241]
See all CD4 primary antibodies -
Description
Biotin Mouse monoclonal [MEM-241] to CD4 -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
CD4 fusion protein (Human).
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General notes
The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-241 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- APC Anti-CD4 antibody [MEM-241] (ab18280)
- FITC Anti-CD4 antibody [MEM-241] (ab18281)
- PE Anti-CD4 antibody [MEM-241] (ab18282)
- APC/Cy7® Anti-CD4 antibody [MEM241] (ab233298)
- PE/Cy7® Anti-CD4 antibody [MEM241] (ab233660)
- Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)
- PerCP Anti-CD4 antibody [MEM-241] (ab65951)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab28069 has been referenced in 2 publications.
- Fennell RD et al. Development of a Microfluidic Device for CD4+ T Cell Isolation and Automated Enumeration from Whole Blood. Biosensors (Basel) 12:N/A (2021). PubMed: 35049640
- Chen YT et al. Quantitatively Resolving Ligand-Receptor Bonds on Cell Surfaces Using Force-Induced Remnant Magnetization Spectroscopy. ACS Cent Sci 2:75-9 (2016). PubMed: 27163031